I'm having a really hard time finding software to help me analyze calcium sparks. I use VSM cells and the fluo-4 am calcium indicator, I record 30s (80 frames) in the LSM780 confocal, each recording contains between 10 to 15 cells or more when I have a vessel fragment. The interesting thing for me initially is counting the number of sparks per cell and doing this manually will take forever plus a tendency to misidentify a spark. Can anyone tell me an easier and more automatic way to do this kind of analysis? Or more appropriate software. Thanks very much in advance.