B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Mixed ANOVA independent variables understanding in SPSS?

I have an experiment where 10 groups of 3 judges are asked to evaluate a list of pictures in 2 evaluation conditions. The pictures were made in 2 between-subjects conditions (A,B) and each group...

20 December 2020 7,697 3 View

How to modelling the concrete rigid slabs in a masonry building ?

I am working on developing an accurate 3D model. When I defined the rigid slab the analyses aborted for the static step. In the Message file appeared this: ***NOTE: THE SOLUTION APPEARS TO BE...

22 July 2020 3,431 2 View

Any suggestion on how to solve problems on DNA gel electrophoresis?

Please find attached a photo of the problem; After a simple Agarose gel electrophoresis of DNA with EtBr i find the gel "divided" in two parts. Analizing at the gel at 259nm it seems that the...

28 April 2020 2,327 7 View

What kind of pharmacological / biological behavior is this?

14 April 2020 9,913 10 View

Can I use "all rights reserved" data for scientific use ?

Hi everyone, I am wondering if I can use "all rights reserved" resources (mainly texts) extracted from the web for testing my models and then (eventually) pubblish a paper. Notice that No data...

29 March 2020 5,026 4 View

Is there exist a tool which extract lexical and synctactical features from a raw text?

Is there exist a tool which extract lexical (e.g average word length, frequency of words) and synctactical features (e.g appositions) from a text?

09 March 2020 4,867 2 View

No title

05 February 2020 4,691 4 View

Harmonics analysis from a control theory perspective?

I was wondering if there are some references available on harmonics analysis (especially for power systems) from a control theory perspective.

08 December 2019 5,996 3 View

How can we conceptualise and explore the relationship between nationalism and climate change?

I have searched some of the top ranking nationalism studies journals (Nations and nationalism, SEN -Studies of ethnicity and nationalism, Nationalism and ethnic politics, Ethnicities,...

28 August 2019 1,344 13 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Why can't make aluminum nitride film successfully by use sputter?

We use reactive sputtering process to make aluminum nitride film, and want to make c-axis aluminum nitride film on a silicon substrate, but the crystal orientation cannot be seen with...

03 March 2021 7,975 3 View

What is the best way to keep an anaerobic chamber anoxic in the absence of hydrogen?

I'm a graduate student who works with an anaerobe, so I often work in an anaerobic chamber. I've been working on an assay that involves the steady-state of the quinone/ quinol pool. Anoxic...

03 March 2021 2,971 1 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

Sutter electrode holder leaking after applying positive pressure. How to fix?

I am using a Sutter electrode holder for my patch clamp experiments, but I'm having trouble keeping positive pressure inside my electrode due to a leak out of the back of the holder. I have added...

02 March 2021 5,829 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

How much pressure we can reduce at the outlet to avoid Reverse Flow?

I am simulating Heat Exchanger. I have tried and tested all the methods to resolve this issue from the internet like refining mesh, improving skewness and orthogonal quality ,extending outlet to...

01 March 2021 6,985 3 View

Exit velocity of natural gas from pressurized tank?

Hi Guys. I'm a mechanical engineer and have a bit trouble, would be very glad if you could help me with my questions. I have a pressurized tank which contains Natural Gas at 200 Bar and 293...

26 February 2021 6,253 3 View