How can I make a preparation of 1M pH 6 potassium dihydrogen phosphate buffer?

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1. Topic ideation and creation Come up with a topic that seeks to solve a local problem in Zimbabwe, social or business problem?

The problem should be supported by evidence such as local news, research work, international focus groups, NGOs, company reports, press conferences etc. Important : Project should solve a local...

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Entropy as a factor in sociological explanations?

How can we apply the second law of thermodynamics to human societies? How can entropy (ie dissipation of energy and/or increase in disorder) be factored in when examining deterioration, change,...

04 March 2024 4,486 7 View

Is healing the soil microbiome effectively linked to promoting human health?

theory: soil microbiome directly influences human gut microbiome and factors of health -> modern, western culture favours and advertises fast food and processed food, which are...

01 October 2023 2,143 2 View

After mixing Streptomycin Sulfate powder in ddH20, the water turned a moderately transparent orange/brown color. Is this normal?

After I mixed Streptomycin Sulfate powder (which is white in color) in ddH20, I stored it in 4°C overnight. The next day, it turned what I can best describe as a moderately transparent...

13 September 2022 7,283 0 View

How can I create an automated single particle tracking (SPT) system that can identify intensity over time of two channels (green & red fluorescence)?

I am currently trying to develop a system that will automatically track single particles. I know this has been done before as I am using TrackPy to accomplish this. Specifically, I am examining...

18 October 2021 9,787 2 View

Proper storage of a western blot membrane following visualization with HRP substrate?

I've heard that storage in TBST is acceptable. What specific temperature should I store at? How long is the membrane viable for?

02 June 2021 5,236 1 View

Troubleshooting in Probing for Myc Tag in HEK Cells?

I've been having issues visualizing blots when probing for the Myc tag in HEK cells. I'll list my basic step outline that I follow, maybe that will be informative in picking out something I'm...

02 June 2021 9,235 3 View

How are research paradigms used in evaluation?

I am a student working with educational research and evaluation. I am wondering how research paradigms are used in evaluation. It is my understanding that these fields are related but differ by...

17 May 2021 9,082 4 View

What analysis to run with multiple IVs and DVs?

I have multiple IVs (4 personality traits, age, gender and frequency use of social media and my DV is whether participants contributed to the circulation of fake news online. I had 3 news posts in...

22 March 2021 3,530 5 View

How do you lyse small cells (10 microns) without disrupting the mitochondria?

We consistently isolate intact/functional mitochondria from liver and heart tissue using manual homogenization (to disrupt the cell membrane) followed by differential centrifugation. I am now...

04 November 2019 9,444 1 View

AMAXA Lonza nucleofection lower volume of buffer?

Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?

07 August 2024 4,616 0 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

How to assess the osmotic power of a substance?

Hello, I suspect that a molecule I am using is causing bacteria to experience osmotic shock. What chemical properties should I look for to compare this capability with those of other substances?...

06 August 2024 977 1 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,783 2 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

How to check pH of a high strength hydrogel?

We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...

30 July 2024 942 2 View

EDTA buffer solution?

Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.

29 July 2024 4,374 1 View

Whether i can preserve leaves samples in 2.5% glutaraldehyde in 0.05 M phosphate buffer, pH 7 for coming several weeks for further LM study ?

I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.

29 July 2024 8,560 0 View

Can a shoot-through event of a tri-state digital buffer cause momentary Hi-Z state?

// interested in the difference between floating events and short circuits.

22 July 2024 6,565 0 View

How can EDTA be removed from already extracted DNA samples?

Our lab has extracted bacterial DNA samples using Qiagen DNeasy kit and eluted the samples in AE buffer. However, this buffer contains 0.5mM EDTA not suitable for downstream application. Any ideas...

13 July 2024 1,675 3 View