How can I make a preparation of 1M pH 6 potassium dihydrogen phosphate buffer?

I can't seem to find the answer online or in my lab anywhere.

09 February 2020 8,490 3 View

How do you lyse small cells (10 microns) without disrupting the mitochondria?

We consistently isolate intact/functional mitochondria from liver and heart tissue using manual homogenization (to disrupt the cell membrane) followed by differential centrifugation. I am now...

04 November 2019 9,279 1 View

In your research with OrganoTropisms are you utilizing tissue culture or histology slides?

Tropisms for various organ systems by borreliae have intrigued me for many years. In my research I have utilized autopsy tissues and tissue cultures for examination with Immunohistochemistry and...

17 March 2019 773 1 View

How do hallucinogens work on the brain?

Robin Carhart-Harris, Mendel Kaelen and David Nutt consider a big questionon several levelsDoes self-organised activity in thedefault mode network underlie oursense of self or ego?

07 August 2016 7,296 0 View

Why are we conscious?

07 August 2016 5,818 2 View

Should we have a basic income in the UK?

07 August 2016 1,723 0 View

What system mediates and incorporates all the potentially competing signalling of the brain?

Does the brain work in harmony, for example in a way where signals work up a chain of command and through the way the modularities of the connectome are integrated they are all given a proportion...

07 August 2016 4,341 0 View

The processing power of the brain, way-beyond binary; Could a Turing like machine decode the encryptions of the human brain?

We clearly process information, the neurochemical signatures are a way neuronal communication occurs between modulars of the brain. We have at least 50 neuro communication chemicals, that is...

07 August 2016 4,129 0 View

How can one make a dilution series before determining titer?

11 March 2015 7,875 3 View

Do you remove the Peyer's patches when doing FACS analysis on the mouse colon or include them in the analysis?

I am looking at the impact on the immune system with different treatments. Should I include the Peyer's patches when putting cells through flow cytometry? Or should they be removed and ran separately?

09 September 2014 8,874 5 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

Recalcitrant seed short-mid storage method?

Dear peer scientists, Ultradry storage is not suitable for recalcitrant seeds. However, theoretically, if we can stabilize the structure of the subcellular membrane, as well as glassify the...

25 February 2021 2,442 1 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View

Protocol for membrane extraction E. coli?

Hello! I am interested in determining whether a poly-unsaturated fatty acid I have engineered E. coli to produce is being incorporated into the membrane of the cells or if it is simply a free...

21 February 2021 7,447 2 View

How to visualise pathways from a phosphoproteomics dataset?

I am looking for something similar than Ingenuity Pathway Analysis (IPA) in which I can enter the proteins differentially phosphorylated and I can visualise the pathways these proteins are...

21 February 2021 8,923 4 View