How can I make a preparation of 1M pH 6 potassium dihydrogen phosphate buffer?

I can't seem to find the answer online or in my lab anywhere.

09 February 2020 8,490 3 View

How do you lyse small cells (10 microns) without disrupting the mitochondria?

We consistently isolate intact/functional mitochondria from liver and heart tissue using manual homogenization (to disrupt the cell membrane) followed by differential centrifugation. I am now...

04 November 2019 9,279 1 View

In your research with OrganoTropisms are you utilizing tissue culture or histology slides?

Tropisms for various organ systems by borreliae have intrigued me for many years. In my research I have utilized autopsy tissues and tissue cultures for examination with Immunohistochemistry and...

17 March 2019 773 1 View

Can I simplify a (ols or logit) model by combining a number of variably related variables into an index?

To be more specific: my goal is to demonstrate that the effects of X1 on Y is not entirely accounted for by attitudes towards various out-groups. The latter items include measures of positivity...

21 October 2018 1,830 2 View

How do hallucinogens work on the brain?

Robin Carhart-Harris, Mendel Kaelen and David Nutt consider a big questionon several levelsDoes self-organised activity in thedefault mode network underlie oursense of self or ego?

07 August 2016 7,296 0 View

Why are we conscious?

07 August 2016 5,818 2 View

Should we have a basic income in the UK?

07 August 2016 1,723 0 View

What system mediates and incorporates all the potentially competing signalling of the brain?

Does the brain work in harmony, for example in a way where signals work up a chain of command and through the way the modularities of the connectome are integrated they are all given a proportion...

07 August 2016 4,341 0 View

The processing power of the brain, way-beyond binary; Could a Turing like machine decode the encryptions of the human brain?

We clearly process information, the neurochemical signatures are a way neuronal communication occurs between modulars of the brain. We have at least 50 neuro communication chemicals, that is...

07 August 2016 4,129 0 View

Can I add new items with smaller Ns to a scale composed of items with larger Ns?

I launched an online survey of personal religiosity in April and have since received entries from almost 700 respondents (hoping to reach 1,000+). At approx. the 600th respondent mark, I added...

04 July 2016 4,155 3 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Alternative to mediation analysis?

I am currently working on my dissertation and want to explore the role of competence as a mediator between the effect of colour of clothes on voting intention (focus on Western countries)....

21 February 2021 8,609 1 View

Color of spiro-ometad film?

What does the color of a spiro-ometad film indicate? I got purple, green, red, even black color of the spiro film. Is there anything we can get from the preliminary color analysis(e.g. oxidation...

21 February 2021 1,831 4 View

What is the original citation for the commonly used activity factors for converting basal metabolic rate to total caloric need?

I see the same activity factor (multipliers) in research papers and websites, but I cannot find where they come from. Does anyone know where these originated? I am looking for an original...

18 February 2021 7,635 2 View

Are ZnS nanoparticles moisture sensitive?

Hello Everyone, I tried to form ZnS nanoparticles using the hot injection method and was able to form the same (confirmed by XRD). When I centrifuged, after the reaction I got a kind of white...

17 February 2021 1,709 3 View

Any information about dinitrosalisylic acid reagent end point color stability ?

I work on estimation of alpha amylase activity using dinitrosalisylic acid reagent If i put the reagent to stope the enzymatic reaction and allow the reagent to react with the produced reducing...

17 February 2021 7,402 4 View

Negative result on natural product sample cholinergic assay?

i have been doing cholinergic assay on natural product and for some of the samples, i get negative value for the inhibition activity. can anyone help me explain the meaning of the negative value...

17 February 2021 4,118 1 View

Has anyone performed a colorimetric RT-LAMP assay using Lucigens LavaLAMP kit?

Hi there, my lab recently opted to use LavaLAMP from Lucigen instead of the Warmstart kit from NEB. However, Lucigen has yet to create a colormetric kit for RT-LAMP, and adding phenol-Red (pH...

17 February 2021 5,399 1 View

How do you interpret multiple meta-regression in STATA?

Please could someone recommend a resource that explains performing meta-regression with multiple moderators. I have read the STATA META ANALYSIS REFERENCE MANUAL, but this only gives examples of...

17 February 2021 7,376 1 View

Pile-Raft connection?

Greetings, I was wondering what is the optimal way to connect the reinforcement at the top of the pile (the part that connects to the raft foundation): -The pile bars are left straight and...

16 February 2021 2,007 3 View