How can I make a preparation of 1M pH 6 potassium dihydrogen phosphate buffer?

I can't seem to find the answer online or in my lab anywhere.

09 February 2020 8,490 3 View

How do you lyse small cells (10 microns) without disrupting the mitochondria?

We consistently isolate intact/functional mitochondria from liver and heart tissue using manual homogenization (to disrupt the cell membrane) followed by differential centrifugation. I am now...

04 November 2019 9,279 1 View

In your research with OrganoTropisms are you utilizing tissue culture or histology slides?

Tropisms for various organ systems by borreliae have intrigued me for many years. In my research I have utilized autopsy tissues and tissue cultures for examination with Immunohistochemistry and...

17 March 2019 773 1 View

How do hallucinogens work on the brain?

Robin Carhart-Harris, Mendel Kaelen and David Nutt consider a big questionon several levelsDoes self-organised activity in thedefault mode network underlie oursense of self or ego?

07 August 2016 7,296 0 View

Why are we conscious?

07 August 2016 5,818 2 View

Should we have a basic income in the UK?

07 August 2016 1,723 0 View

What system mediates and incorporates all the potentially competing signalling of the brain?

Does the brain work in harmony, for example in a way where signals work up a chain of command and through the way the modularities of the connectome are integrated they are all given a proportion...

07 August 2016 4,341 0 View

The processing power of the brain, way-beyond binary; Could a Turing like machine decode the encryptions of the human brain?

We clearly process information, the neurochemical signatures are a way neuronal communication occurs between modulars of the brain. We have at least 50 neuro communication chemicals, that is...

07 August 2016 4,129 0 View

How can one make a dilution series before determining titer?

11 March 2015 7,875 3 View

Do you remove the Peyer's patches when doing FACS analysis on the mouse colon or include them in the analysis?

I am looking at the impact on the immune system with different treatments. Should I include the Peyer's patches when putting cells through flow cytometry? Or should they be removed and ran separately?

09 September 2014 8,874 5 View

What are some of the research gaps in machine learning and artificial intelligence in africa?

i would to know some of the research gaps in the artificial intelligence field in most african countries.

03 March 2021 6,145 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

No title

01 March 2021 3,905 5 View

Which group formation theory do you recommend for projects that aim to bring together entrepreneurs for training for regional development?

I would like to have ideas about which theories of group formation are suitable for entrepreneurial training projects, with the aim of creating bonds, building trust, and collaboration for...

28 February 2021 4,223 2 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

What is the optimum time and temperature of blanching and pre-frying potato for frozen fries?

I am helping a local home industry for developing frozen fries from their local farm. I want to know what is the optimum blanching and pre-frying temperature and time for processing. Please also...

23 February 2021 1,616 5 View

How to find out the problem of VMD software when i run it?

Hello I try to install and run the VMD software on windows 10 (64-bit).It doesn`t work and it is closed when I run it. I checked all of tutorials, videos and documents. But they did not work...

22 February 2021 8,211 3 View

What to do with absence of fluorescence label in Fucci2a mouse-derived fibroblast culture?

Dear all, I have harvested fibroblast cells from R26p-Fucci2 mouse embryos (mouse line as described in this paper: https://dev.biologists.org/content/140/1/237) and cultured them on plastic,...

17 February 2021 3,368 2 View