Do I have to culture the cells in a solid matrix and make confucal image? Would some one provide me a protocol for this?
Use the single picture files and then HeliconSoft Helicon Focus to make the 3D picture.
What exactly is Retrieval Augmented Generation for Large Language Model doing? Isn’t it data engineering?
30 July 2024 7,376 3 View
After a lot of feature engineering for click-through rate modeling, it feels like it's basically the end of iteration? I mean, it's not cost-effective to keep doing it?
29 July 2024 4,955 0 View
all math can be traversed by code? all math can be translate to code?
26 July 2024 9,530 0 View
What is the effect for the CTR model, adding the tag-id with the highest number of user clicks/purchases on the item's tag as user-side features?
22 June 2024 3,588 1 View
The primary problem that large language models solved is small sample learning, right?
03 June 2024 3,305 2 View
based on GPT-3
01 June 2024 5,445 3 View
Or they are only complement to each other
01 June 2024 6,021 3 View
For example, if offline click-AUC improves from 0.77 to 0.82 VS pay-AUC improves from 0.88 to 0.91, which online gain will be greater?
21 May 2024 3,563 1 View
It seems it is also through several dimensions.
13 May 2024 8,099 3 View
Swin-Transformer transform the image to tokens to input to transformer. Is each token (before-embedding) value an integer? In practice, where is this done?...
13 May 2024 1,121 2 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View
what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...
03 August 2024 5,828 4 View