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Questions related from Dan Cacsire Castillo-Tong
DNA replication in living organisms need RNA primers to initiate. How long are these primers and what kind of other characteristics do these primers have? Where and how are they produced? Are they...
11 November 2019 2,486 3 View
By chemosensitivity test of cell lines to carboplatin, different exposure time of cells to the drug were described in the literature, typically 3-4 days. Very rarelly, there were also tests with...
09 September 2015 8,852 6 View
Can anyone tell me how to achieve the deviation of NGS for gene expression profiling? In our case, TruSeq RNA and DNA samples Preparation Kits v2 and illumina were useless.
10 October 2014 5,483 6 View
Genetic changes accumulate in tumor cells causing the formation of coexistence of different clones of cells, which manage to survive. When culturing cells in vitro, the numbers of chromosomes are...
09 September 2014 7,201 5 View
We quite often observe triploid clones of tumor cells. How can a triploid happen by mitosis?
08 August 2014 7,120 25 View
Does anyone know a magnetic stirrer, which can be used for cell culture in the incubator? Optimistically, small and with battery power and slow rotation.
03 March 2013 6,056 1 View
We are trying to establish primary culture from ascites and tumor tissues from ovarian cancer patients. Yesterday, we got these materials from one patient, from whom we still have not got the...
02 February 2013 3,985 17 View
Do I have to culture the cells in a solid matrix and make confucal image? Would some one provide me a protocol for this?
10 October 2012 6,728 1 View
10 October 2012 7,393 4 View
We are trying to establish primary cell culture from ovarian tumors or ascites. At the beginning, most epithelial-like cells grow in cluster, forming a gland-like structure, which seems to be...
10 October 2012 9,372 11 View
