What's the optimal temp. for hot start Taq polymerase? and do we need an special PCR condotion or not?

I've ran a PCR using Hot start Taq polymerase instead of normal taq polymerase by mistake, but as the result i have found some pure and non-sharp bands, may any one help me to find why it has...

11 December 2019 4,912 3 View

What's the effective protocol for DNA extraction from Filamentous fungi?

I have tried some protocols in order to extracting DNA from Filamentus fungi, such as proteinase K, RNxplus, TRizol, 95 Degrees of c. heat and ....but still its a failure. how should I extract...

06 July 2016 5,620 9 View

In the case of circulating miRNAs expression measurement, which one is more credible? the Serum or Plasma?

03 April 2016 4,143 4 View

Housekeping Genes: which one is the best?

there are some genes accepted as internal control for gene expression analyses but nowadays some researchers say expression of these housekeeping genes are varying from a tissue to other or their...

05 June 2015 5,485 8 View

What are the sings of necrosis?

is it ok to say necrosis will increase the activation and expression of anti-apoptotic genes and pathways? I have some problem in my project which lead me to think that necrosis will increase the...

03 April 2015 4,161 4 View

How can I find the related lncRNAs of a gene?

I'm looking for regulators of a specific gene, want to find sncRNA ( like microRNAs) regulators and LncRNA regulators of the gene... about the microRNAs I've find some useful info using miRWalk...

02 March 2015 8,876 4 View

Has anyone extracted DNA from cultured apoptotic cells using TRIzol kit?

I had extracted total RNA of cells according to the manufactures and tried to extract DNA of the same sample respectively, but i see no bound on electrophoresis gel. I followed all steps one by...

02 March 2015 2,836 5 View

Should I centrifuge the cells after thawing them in order to remove the DMSO from the medium?

All we know, centrifuging right after thawing the cells is harmful. But in the other hand, remaining the DMSO in the medium can hit the cells, too. So shall I remove the DMSO or it's better to...

11 December 2014 1,311 23 View

How much FBS do I need to add to the medium in order to prevent cell division in 96-well plates?

My MCF-7 cells are dividing in 5% medium, but I need to keep them alive and prevent them from division...

08 September 2014 1,457 4 View

How to calculate the IC50 for my MTT assay?

there is many formulas for calculating the IC50 value but I can not use them because I don't know what does mean x, x', Y and Y'. there is a formula Y = a * X + b, IC50 = (0.5 - b)/a. but I don't...

07 August 2014 4,404 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View