How can I isolate the blood plasma without degradation, precipitation and/or loss of microRNAs of the blood?

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What's the optimal temp. for hot start Taq polymerase? and do we need an special PCR condotion or not?

I've ran a PCR using Hot start Taq polymerase instead of normal taq polymerase by mistake, but as the result i have found some pure and non-sharp bands, may any one help me to find why it has...

11 December 2019 5,062 3 View

What's the effective protocol for DNA extraction from Filamentous fungi?

I have tried some protocols in order to extracting DNA from Filamentus fungi, such as proteinase K, RNxplus, TRizol, 95 Degrees of c. heat and ....but still its a failure. how should I extract...

06 July 2016 5,738 9 View

In the case of circulating miRNAs expression measurement, which one is more credible? the Serum or Plasma?

I need to evaluate the level of microRNAs in human blood, but I'm not sure which one is more credible, the plasma or serum. I would be very grateful if some one helps me? besides, as you know...

03 April 2016 4,257 4 View

Housekeping Genes: which one is the best?

there are some genes accepted as internal control for gene expression analyses but nowadays some researchers say expression of these housekeeping genes are varying from a tissue to other or their...

05 June 2015 5,603 8 View

What are the sings of necrosis?

is it ok to say necrosis will increase the activation and expression of anti-apoptotic genes and pathways? I have some problem in my project which lead me to think that necrosis will increase the...

03 April 2015 4,280 4 View

How can I find the related lncRNAs of a gene?

I'm looking for regulators of a specific gene, want to find sncRNA ( like microRNAs) regulators and LncRNA regulators of the gene... about the microRNAs I've find some useful info using miRWalk...

02 March 2015 9,034 4 View

Has anyone extracted DNA from cultured apoptotic cells using TRIzol kit?

I had extracted total RNA of cells according to the manufactures and tried to extract DNA of the same sample respectively, but i see no bound on electrophoresis gel. I followed all steps one by...

02 March 2015 2,968 5 View

Should I centrifuge the cells after thawing them in order to remove the DMSO from the medium?

All we know, centrifuging right after thawing the cells is harmful. But in the other hand, remaining the DMSO in the medium can hit the cells, too. So shall I remove the DMSO or it's better to...

11 December 2014 1,739 23 View

How much FBS do I need to add to the medium in order to prevent cell division in 96-well plates?

My MCF-7 cells are dividing in 5% medium, but I need to keep them alive and prevent them from division...

08 September 2014 1,641 4 View

How to calculate the IC50 for my MTT assay?

there is many formulas for calculating the IC50 value but I can not use them because I don't know what does mean x, x', Y and Y'. there is a formula Y = a * X + b, IC50 = (0.5 - b)/a. but I don't...

07 August 2014 4,591 0 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View