What's the optimal temp. for hot start Taq polymerase? and do we need an special PCR condotion or not?

I've ran a PCR using Hot start Taq polymerase instead of normal taq polymerase by mistake, but as the result i have found some pure and non-sharp bands, may any one help me to find why it has...

11 December 2019 4,912 3 View

What's the effective protocol for DNA extraction from Filamentous fungi?

I have tried some protocols in order to extracting DNA from Filamentus fungi, such as proteinase K, RNxplus, TRizol, 95 Degrees of c. heat and ....but still its a failure. how should I extract...

06 July 2016 5,620 9 View

In the case of circulating miRNAs expression measurement, which one is more credible? the Serum or Plasma?

03 April 2016 4,143 4 View

Housekeping Genes: which one is the best?

there are some genes accepted as internal control for gene expression analyses but nowadays some researchers say expression of these housekeeping genes are varying from a tissue to other or their...

05 June 2015 5,485 8 View

What are the sings of necrosis?

is it ok to say necrosis will increase the activation and expression of anti-apoptotic genes and pathways? I have some problem in my project which lead me to think that necrosis will increase the...

03 April 2015 4,161 4 View

Has anyone extracted DNA from cultured apoptotic cells using TRIzol kit?

I had extracted total RNA of cells according to the manufactures and tried to extract DNA of the same sample respectively, but i see no bound on electrophoresis gel. I followed all steps one by...

02 March 2015 2,836 5 View

Should I centrifuge the cells after thawing them in order to remove the DMSO from the medium?

All we know, centrifuging right after thawing the cells is harmful. But in the other hand, remaining the DMSO in the medium can hit the cells, too. So shall I remove the DMSO or it's better to...

11 December 2014 1,311 23 View

How much FBS do I need to add to the medium in order to prevent cell division in 96-well plates?

My MCF-7 cells are dividing in 5% medium, but I need to keep them alive and prevent them from division...

08 September 2014 1,457 4 View

How to calculate the IC50 for my MTT assay?

there is many formulas for calculating the IC50 value but I can not use them because I don't know what does mean x, x', Y and Y'. there is a formula Y = a * X + b, IC50 = (0.5 - b)/a. but I don't...

07 August 2014 4,404 0 View

What kind of contamination do you think this could be?

07 August 2014 1,373 17 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View

Why does two different bioinformatics tools for the same proteins show different subcellular localizations?

During a computational analysis, I found that for a protein of a plant, two different subcellular localizations are seen using CELLO and WoLF PSORT. For example, in CELLO, it identified the...

01 March 2021 6,729 2 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

How can we simulate an hyperelastic-viscoelastic behavor with Ansys APDL?

I am working on modeling and simulation of biomecanical material behaviour, I have succeded on simulating skin using anisotropic hyperelastic material, on APDL and in our lab's finite element...

28 February 2021 552 3 View