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Questions related from Aref Sobhkhizy
I've ran a PCR using Hot start Taq polymerase instead of normal taq polymerase by mistake, but as the result i have found some pure and non-sharp bands, may any one help me to find why it has...
12 December 2019 5,034 3 View
I have tried some protocols in order to extracting DNA from Filamentus fungi, such as proteinase K, RNxplus, TRizol, 95 Degrees of c. heat and ....but still its a failure. how should I extract...
07 July 2016 5,692 9 View
I need to evaluate the level of microRNAs in human blood, but I'm not sure which one is more credible, the plasma or serum. I would be very grateful if some one helps me? besides, as you know...
04 April 2016 4,215 4 View
there are some genes accepted as internal control for gene expression analyses but nowadays some researchers say expression of these housekeeping genes are varying from a tissue to other or their...
06 June 2015 5,565 8 View
is it ok to say necrosis will increase the activation and expression of anti-apoptotic genes and pathways? I have some problem in my project which lead me to think that necrosis will increase the...
04 April 2015 4,235 4 View
I had extracted total RNA of cells according to the manufactures and tried to extract DNA of the same sample respectively, but i see no bound on electrophoresis gel. I followed all steps one by...
03 March 2015 2,921 5 View
I'm looking for regulators of a specific gene, want to find sncRNA ( like microRNAs) regulators and LncRNA regulators of the gene... about the microRNAs I've find some useful info using miRWalk...
03 March 2015 8,994 4 View
All we know, centrifuging right after thawing the cells is harmful. But in the other hand, remaining the DMSO in the medium can hit the cells, too. So shall I remove the DMSO or it's better to...
12 December 2014 1,613 23 View
My MCF-7 cells are dividing in 5% medium, but I need to keep them alive and prevent them from division...
09 September 2014 1,598 4 View
12 hours after I've passaged my MCF7 cells I found out this unique cell which is disappeared now. Is it fungal contamination or something else?
08 August 2014 1,434 17 View
Every time I wash cells, they began to detaching and get circular for some minutes and after replacing the medium they reattach again, does anyone please have idea to stop it?I have tried to keep...
08 August 2014 9,235 5 View
Can anyone please suggest how many MCF 7 cells do I need in each well for optimized mtt assay? as you know, growth rate of mcf-7 cells is very high. is 5000 cells enough for each well ?
08 August 2014 10,046 3 View
there is many formulas for calculating the IC50 value but I can not use them because I don't know what does mean x, x', Y and Y'. there is a formula Y = a * X + b, IC50 = (0.5 - b)/a. but I don't...
08 August 2014 4,520 0 View
I need to thaw the frozen MCF7 and T47D cell lines but they are frozen for more than 6 months and I still worry about the storage conditions and percent of alive cells, any tips for the best...
07 July 2014 7,754 34 View
I have isolated some blood microRNAs. Can I directly use these miRNAs as a kit to suppress a gene of interest in my cultured cells? Can we use isolated circulating microRNAs to control the...
04 April 2014 7,957 15 View
I'm looking for the best performance to extract RNA from some tissues which are stored for more than 1 year or even a couple of years in - 80 degrees of Celsius and we are not sure about its...
04 April 2014 1,883 10 View
What's the best protocol to isolate the blood plasma in order to save all microRNAs of the fresh blood?
04 April 2014 8,298 2 View
Can anyone please help me to find the most common form of a SNP of a gene? Also I don't know the most common rs numbers of the SNPs. I know how to find the rs numbers but my problem is that I...
04 April 2014 10,004 4 View
While all RNAs are sensitive to denaturation, why are microRNA stable?
03 March 2014 2,710 4 View
As we know pri-miRNA contains two strands, which one of them is (exactly or partly) complementary to the target mRNA (antisens or miRNA) and the other one is (exactly or partly) the same as a...
03 March 2014 9,945 4 View
As we know pri-miRNA contains two strands, which one of them is (exactly or partly ) complementary to target mRNA (antisens - miRNA) and the other one is (exactly or partly ) same as a sense...
03 March 2014 1,536 6 View
How do miRNA control the methylation? Is there any acceptable theory?
03 March 2014 3,212 3 View
I need a primer for some genes without introns and also it should check the DNA contamination to ensure there is no DNA contamination. For normal genes we can use intron junctions to check (or...
03 March 2014 7,607 6 View
I have to deliver two different siRNA at the same time to cancerous cell culture whilst satisfying efficiency.
01 January 2014 2,759 8 View
I'm looking for exact information even like si-RNA sequences and how to work on it.
01 January 2014 2,224 8 View
How TAg and tAg of BK virus induce tumor cells?
12 December 2013 8,232 4 View
