How to calculate the IC50 for my MTT assay?

08 August 2014 0 5K Report

there is many formulas for calculating the IC50 value but I can not use them because I don't know what does mean x, x', Y and Y'.

there is a formula

Y = a * X + b,

IC50 = (0.5 - b)/a.

but I don't know how to use it

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Science method

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