I developed drug-loaded chitosan nanoparticles using TPP as a crosslinker with the ionotropic gelation technique. I dissolved the drug in water and added it to the 1% (w/v) chitosan solution. I added this solution dropwise to the 0.8% TPP solution and beads were formed. After centrifugation, I discarded the supernatant and dried the pellet by lyophilization. But the pellet I obtained is not soluble in water or 1% acetic acid solution. How can we dissolve the drug-loaded chitosan nanoparticles we obtained for the MIC and time-kill experiments? Or does the drug need to be insoluble for sustained drug release? Can you help with this issue?
Irfan Akartas
Prepare a solution of hydrochloric acid with a pH of 1.5-2, as in the stomach. If the shell of your drug partially dissolves in this solution, then you have created a drug with a prolonged release.
Yuri Mirgorod chitosan nanoparticles dissolved partially in 1% acetic acid solution but were not fully dissolved during 24 hours. Also after the in vitro drug release tests, it showed prolonged release. Should I have to dissolve it completely to perform the activity evaluation such as MIC or time-kill assay for antibacterial activity? Or is it enough for me to apply it only to the medium and wait for the drug release, depending on time, since it shows prolonged release?
Irfan Akartas
Apply it only to the medium and wait for the release of the drug depending on the time
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