How can I dissolve C8 ceramide, C2 ceramide, galactosyl and clucosyl ceramide?

More Koushik Mondal's questions See All

What is the population percentage of Transitional B cell subsets (T1, T2 and T3) in normal condition?

I want to know how the population of Transitional B cell subsets varies with age in mice or in human during normal condition

02 March 2016 3,877 2 View

How much splenocyte/lymphocytic cells do I need for 6 well/12 well /24 well for B cell mediated IL-10 secretion assay standardization?

I want to observe IL10 secretion by lymphocytic cells, could anyone of you guys suggest to me how much splenocytic/lymphocytic cell I need to plate for 6 well/12 well/24 well plates

02 March 2015 6,399 7 View

Which is appropriate for blocking B cell activation, Monensin or Brefeldin A?

I want to test IL10 secretion from B lymphocyte. Do I need to use Monensin or Brefeldin A for blocking? Kindly clarify whether or not Monensin is a B lymphocyte blocker and Brefeldin A is for T...

02 March 2015 8,864 6 View

How can I measure the lambda kappa ratio during autoimmunity?

I want to identify the loss of receptor editing during a Leishmania infection, that's why I like to understand the lambda kappa ratio. Could anyone provide the protocol for this experiment, please?

09 October 2014 2,535 3 View

Why light chain of Immunoglobulin has lambda and kappa locus, but for heavy chain only one?

The gene rearrangement of light chain is in either kappa or lambda, whereas heavy chain dnt have two locus, can anyone provide me the exact reason behind this?

09 October 2014 4,554 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View