What is the population percentage of Transitional B cell subsets (T1, T2 and T3) in normal condition?

I want to know how the population of Transitional B cell subsets varies with age in mice or in human during normal condition

02 March 2016 3,800 2 View

How much splenocyte/lymphocytic cells do I need for 6 well/12 well /24 well for B cell mediated IL-10 secretion assay standardization?

I want to observe IL10 secretion by lymphocytic cells, could anyone of you guys suggest to me how much splenocytic/lymphocytic cell I need to plate for 6 well/12 well/24 well plates

02 March 2015 6,342 7 View

Which is appropriate for blocking B cell activation, Monensin or Brefeldin A?

02 March 2015 8,785 6 View

How can I measure the lambda kappa ratio during autoimmunity?

09 October 2014 2,448 3 View

Why light chain of Immunoglobulin has lambda and kappa locus, but for heavy chain only one?

The gene rearrangement of light chain is in either kappa or lambda, whereas heavy chain dnt have two locus, can anyone provide me the exact reason behind this?

09 October 2014 4,474 0 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View