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Questions related from Koushik Mondal
I want to work on some cell culture based experiment, could anyone suggest me how they dissolve C8 ceramide, glucosyl ceramide, galactosyl ceramide,
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I want to know how the population of Transitional B cell subsets varies with age in mice or in human during normal condition
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I want to observe IL10 secretion by lymphocytic cells, could anyone of you guys suggest to me how much splenocytic/lymphocytic cell I need to plate for 6 well/12 well/24 well plates
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I want to test IL10 secretion from B lymphocyte. Do I need to use Monensin or Brefeldin A for blocking? Kindly clarify whether or not Monensin is a B lymphocyte blocker and Brefeldin A is for T...
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The gene rearrangement of light chain is in either kappa or lambda, whereas heavy chain dnt have two locus, can anyone provide me the exact reason behind this?
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I want to identify the loss of receptor editing during a Leishmania infection, that's why I like to understand the lambda kappa ratio. Could anyone provide the protocol for this experiment, please?
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