Koushik Mondal

6 Questions 9 Answers 0 Followers

Questions related from Koushik Mondal

How can I dissolve C8 ceramide, C2 ceramide, galactosyl and clucosyl ceramide?

I want to work on some cell culture based experiment, could anyone suggest me how they dissolve C8 ceramide, glucosyl ceramide, galactosyl ceramide,

09 September 2017 8,947 3 View

What is the population percentage of Transitional B cell subsets (T1, T2 and T3) in normal condition?

I want to know how the population of Transitional B cell subsets varies with age in mice or in human during normal condition

03 March 2016 3,850 2 View

How much splenocyte/lymphocytic cells do I need for 6 well/12 well /24 well for B cell mediated IL-10 secretion assay standardization?

I want to observe IL10 secretion by lymphocytic cells, could anyone of you guys suggest to me how much splenocytic/lymphocytic cell I need to plate for 6 well/12 well/24 well plates

03 March 2015 6,379 7 View

Which is appropriate for blocking B cell activation, Monensin or Brefeldin A?

I want to test IL10 secretion from B lymphocyte. Do I need to use Monensin or Brefeldin A for blocking? Kindly clarify whether or not Monensin is a B lymphocyte blocker and Brefeldin A is for T...

03 March 2015 8,833 6 View

Why light chain of Immunoglobulin has lambda and kappa locus, but for heavy chain only one?

The gene rearrangement of light chain is in either kappa or lambda, whereas heavy chain dnt have two locus, can anyone provide me the exact reason behind this?

10 October 2014 4,518 0 View

How can I measure the lambda kappa ratio during autoimmunity?

I want to identify the loss of receptor editing during a Leishmania infection, that's why I like to understand the lambda kappa ratio. Could anyone provide the protocol for this experiment, please?

10 October 2014 2,504 3 View