The gene rearrangement of light chain is in either kappa or lambda, whereas heavy chain dnt have two locus, can anyone provide me the exact reason behind this?
I want to work on some cell culture based experiment, could anyone suggest me how they dissolve C8 ceramide, glucosyl ceramide, galactosyl ceramide,
08 September 2017 8,978 3 View
I want to know how the population of Transitional B cell subsets varies with age in mice or in human during normal condition
02 March 2016 3,877 2 View
I want to observe IL10 secretion by lymphocytic cells, could anyone of you guys suggest to me how much splenocytic/lymphocytic cell I need to plate for 6 well/12 well/24 well plates
02 March 2015 6,399 7 View
I want to test IL10 secretion from B lymphocyte. Do I need to use Monensin or Brefeldin A for blocking? Kindly clarify whether or not Monensin is a B lymphocyte blocker and Brefeldin A is for T...
02 March 2015 8,864 6 View
I want to identify the loss of receptor editing during a Leishmania infection, that's why I like to understand the lambda kappa ratio. Could anyone provide the protocol for this experiment, please?
09 October 2014 2,535 3 View
Approximate concentrations are require in compared with the WHO permissible limts
11 August 2024 2,723 1 View
I used eye tracking to examine how participants from two different populations (A and B) react to an image. Participants in population A exhibit larger pupil sizes over time, but they also have...
10 August 2024 3,229 0 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...
05 August 2024 10,079 0 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
Read the journal article by Douglas M. Lambert, “The Eight Essential Supply Chain Management Processes,” Supply Chain Management Review, Vol. 8, No. 6 (2004), pp. 18-26
04 August 2024 9,919 4 View