I want to know how the population of Transitional B cell subsets varies with age in mice or in human during normal condition
it is strain dependent, and also housing dependent. This must be tested independent.
to do the experiment by own, follow the multicolor staining protocol as follows,
Get these copies
FOR Mouse: B cell protocol book: Chapter by Richard R. Hardy.
FOR Human: Human Peripheral Blood B-Cell Compartments:
A Crossroad in B-Cell Traffic, 2010, Cytometry Part B (Clinical Cytometry) 78B (Suppl. 1):S47–S60 (2010)
Human, T1 from blood sampling is challenging to conclude.
Try this one dear in addition
My best wishes
Article Transitional B cell subsets in human bone marrow
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