How can I determine if a GABA potentiator, administered to pregnant mice at E15, with subsequent embryonic brain dissection 3 hours later and fixed tissue processing, has affected neural activity, given that standard c-Fos immunohistochemistry (IHC) has been unsuccessful?
Being a regulator, and that embryonic tissues is perfunctory, a culture is needed for three hours, 800 samples may be required, the plot is static. A culture which inhibits the potentiator and then maybe 450 cycles. remove the culture and assess perfunctory capacity for 200 cycles. Register three plots then revise immuno-regulators, and assess disposal for fifty cycles. Consider in extended trials peripotent, conservation, and priori administration the routine is then 160:50:18:12. Considerations embryonic sack failure, oocyte regulation, formation of peripotent curve. The embryo will reject nutrients, and the tissue will have stronger immune function. This concept is right sided, it is up to trials to potentiate a left sided argument.
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