How to separate tannins from poylsaccharides ?

How to extract tannins from a plant without polysaccharides ? With hot water extraction , which seems to be optimal conditions to extract tannins, we also extract a lot of polysaccharides. Is...

06 April 2020 5,271 6 View

Will Maxent discard presence points if some environmental data are missing?

Hi I have a csv file of 160 presence points. Of these points, 5 are missing environmental data in one layer (mean annual precipitation). All layers have same extent and resolution (pixels = 300 m...

26 February 2020 8,621 5 View

Why are my response curves so different in Maxent?

Hi, I used Maxent to create a model identifying the best habitats for the Short-eared Owl. I used a 800mX800m grid (0,5 by 0,5 miles). I calculated, for each environmental variable, the...

03 February 2020 2,232 6 View

Spin columns: difference between those for plasmids, pcr product, virus and genomic DNA??

Hi! I've been looking for replacement spin columns for purifying PCR product from 16s bacterial DNA. I have found several "different" ones online: some for plasmid DNA, some for genomic DNA, some...

19 July 2018 1,125 4 View

Mammalian mtDNA isolation: sucrose gradient or spin columns?

Hi! I've been trying to isolate complete mtDNA plasmids from cheek cells (16.5kbp). Once the nuclear DNA is pelleted and the supernatant containing the mtDNA is transfered in a new tube, pelleted,...

25 May 2018 231 3 View

DNA extraction protocol for anaerobic bacteria? (Gram + and -)?

Hi! I have been trying to extract DNA from anaerobic bacteria (both gram positive and negative) using the lyzozyme/proteinase K/sds/chloroform/isopropanol protocol, but have repeatly failed to get...

26 April 2018 7,323 4 View

Pharmacia EPS 3500 XL electrophoresis power supply leads?

Hi! Ok, that may be a very basic question to ask this community, but I have searched and searched and searched some more, and can't seem to find the answer! What are the required adapters to...

21 April 2018 4,354 0 View

Where can i purchase the L-02 cell line ?

We need the L-02 cell line for our research purpose. Does anyone have a correct contact address for this cell line? Thanks

05 October 2016 9,612 1 View

Paramecium and electricity: why do they stop responding to galvanotaxis after a while?

Hi, I am working on an interactive art installation that offers contact zones between human and non-human. In this specific case, my non-human are Paramecium Caudatum and Multimicronucleatum. I am...

27 March 2016 9,298 5 View

Does anybody have experience in fixing fishbrain in PFA without doing a perfusion?

I am going to fixate some California killifish brains in PFA, but will not be able to do a Cardiac perfusion. Does anybody know if it will be possible to Place the Whole scull (or the Whole brain)...

21 March 2016 5,116 2 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

I have designed reflectarray element and feeding it though Horn Antenna on HFSS. After full wave simulation, I am not getting desired results?

I have designed reflectarray element to generate OAM beams at 5.8GHz and feeding it though Horn Antenna on HFSS. After full wave simulation, i am not getting desired results i.e Radiation pattern...

01 March 2021 9,701 5 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View