Hello everyone, I am just starting my literature review about the subject and I cannot find an answer to that question. From all I know, no study seems to take the enzyme activity into account when they do stomach content analysis. I must have not understood something basic about that because I know most digestives enzymes are not denatured by freezing them. It does not seem to be a problem in studies that do metabarcoding after visual analysis of stomach content but you always want to keep the specimen as much preserve as you can to avoid DNA degradation.
Thank you for your help.