I have got some IF results using LSM, and I got the merge results on that computer. However, what software can I use to conform all the result and get a total result in one picture? Can someone get my idea? Thank you.
you can download for free LSM image Browser or ImageJava to open and analyse your photos. In both you can open stacks separately for chanells, separately for layer..... but Image J gives more tools to measure and analyse your photos.
you can download for free LSM image Browser or ImageJava to open and analyse your photos. In both you can open stacks separately for chanells, separately for layer..... but Image J gives more tools to measure and analyse your photos.
ImageJ is a really good program (free download from NIH website, as Marcelo mentioned). It allows you to merge channels or analyze them separately, do cell count, pixel intensity, and many other types of analyses.
ImageJ is the program to use. To open files from differential microscopes one has to use the LOCI Bio-Formats importer ( http://loci.wisc.edu/software/bio-formats ) or even better just get the fully loaded Fiji (Fiji is just ImageJ; http://fiji.sc/Fiji)