06 June 2013 4 8K Report

I am culturing by 1640 basis medium (HEPES added, Glu added) + 10% FBS + 1% PS +0.1% β-ME, which taught by my colleague. Somehow, there is no one to freeze the THP-1 and I have searched many different formula medium for freezing THP-1.

Do I need to calculate the cell density before freezing them?

What is the optimal formula medium for freezing THP-1 cell? Can I make use of the freezing medium included 60%1640+30%FBS+10%DMSO? Or should I decrease the 10%DMSO to 5%DMSO? Did the 1640 medium is the basis one, without FBS and PS?

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