15 Questions 27 Answers 0 Followers
Questions related from Miao Lee
Hello, everyone In my current real-time PCR assay, I saw the beta-actin was not stable in the LPS-stimulation cells for longer time. It seems the internal control gene-beta-actin is not always...
05 May 2016 2,330 4 View
Hello everyone, I have had two same clones by using different cDNA from different cells. Before the sequencing, I got single clone of DH5-a bacteria and have PCR, restriction enzyme to confirm...
05 May 2015 1,575 3 View
Hey, everyone, My PCR results with two bright binds puzzeled me these days. My primers are designed by Primer-Blast of NCBI and compared to mRNA-Ref database, the expected band is 200bp....
04 April 2015 9,904 10 View
Hello, Have someone used FITC-dextran to measure the phagocytosis of macrophagy? Can you give me a protocol about the working concentration and time? Why I can not get the result along with...
11 November 2014 870 10 View
I wanted to test my cells ROS production by Reactive Oxygen Species (ROS) Detection Reagents (invitrogen), but I did not get positive results even with my control (PMA treated), ROS is lower than...
05 May 2014 6,920 10 View
I am doing research on autophagy with bacteria. According to our lab protocol, first we leave the cells (RAW264.7) infected with bacteria for an hour at MOI=10, then wash out the cells in order to...
04 April 2014 4,110 2 View
I have got some IF results using LSM, and I got the merge results on that computer. However, what software can I use to conform all the result and get a total result in one picture? Can someone...
03 March 2014 2,679 8 View
I have used THP-1 and RAW cell line to confirm a result, however, the results differ from each other. Is this because that my THP-1 cell line was not derived from monocytes to macrophages?
01 January 2014 8,388 4 View
I have transfected GFP-tag plasmid to THP-1 cells, and hope to check the efficiency. I used 24-well and there were 5*10^5 cells per well. I used 50ul opti-MEM mixed with 1ul lipo, and used another...
01 January 2014 1,117 4 View
Now, i am going to find the ligand of molecular X. We are preparing to overexpress the molecular X in cells , and get the cell membrane to co-IP. But someone has told me the ligand and the...
06 June 2013 5,530 11 View
I am culturing by 1640 basis medium (HEPES added, Glu added) + 10% FBS + 1% PS +0.1% β-ME, which taught by my colleague. Somehow, there is no one to freeze the THP-1 and I have searched many...
06 June 2013 7,556 4 View
I want to do the plasmid midi research by 25ml DH5α-plasmid, but the concentration i got is very low, about 10 ng/ul. I followed the protocol every step, and i don't know the reason. Can i...
05 May 2013 9,613 3 View
I diluted the DNA templates 10 fold until the smallest one has 1 copy and 5 copies. Then I do q-PCR with following system: SYBR 5ul F primer 0.4ul Rprimer 0.4ul cDNA 1ul H2O...
03 March 2013 686 4 View
As we all known, the plasmid map point out some characteristics of the plasmid. We should choose the palsmid depending on the plasmid map. But, I really don't know how to read and get more...
03 March 2013 3,333 4 View
My PCR outcome has something wrong, the band smeared seriously and I cannot find the reason. (1
02 February 2013 7,737 17 View
