Which Promotor Sites can I use for In Vitro Transcription?

27 February 2024 1 2K Report

Our lab is trying to use in vitro transcription to create mRNA of our inserted on a pcDNA 3.4 TOPO plasmid. I noticed it does not have a T7 promotor sequence. Are there other available promotors on the market we could use for in vitro transcription?

Laura Leighton

The other IVT polymerase commonly used for molecular biology applications is SP6. I had a look at your plasmid backbone though, and I'm pretty sure it won't have the SP6 promoter either. I think you will need to clone your cDNA into an IVT vector for this experiment to work.

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