Hi all,
I am currently trying to analyze mRNA expression of mitochondrial encoded genes in musculus gastrocnemius of rats. Concerning primer design I designed my primers with the mtichondrion complete genome FASTA by NCBI, because as far as I understood, mitochondrial DNA has almost non introns, meaning the complete genome sequence should be almost the same as the mRNA sequence. Further on I entered the range in which the gene of my interest was located and so I got several primers as a result. Now I just wanted to know, if this is the right way to design primers for mitochondrial encoded genes or if you have any suggestions for me.
Thanks a lot!
Dear
Be cautious on the point that mitochondria genes have no introns. Literatures suggest otherwise.
Best.
see also:https://www.researchgate.net/post/Designing_primers_for_mitochondrial_transcripts
most animal mtDNAs have no introns.
The primers should work, just be carefull with the location, because polycistronic transcripts are cleaved.
Article The Human Mitochondrial Transcriptome
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