Does anyone have experience on the PhiX concentration required on NovaSeq for ddRAD or RADseq data?

Dear all, we have been running multiple ddRAD libraries prepared by following the protocol of Petersen et al 2014 in our lab on the HiSeq2500 (SR 100) in the past couple of years. We pooled...

28 September 2020 2,297 1 View

Read counts from RNA-Seq paired end reads?

Hello, I have the following question on paired end reads from RNA-seq: To get the total number of obtained reads from paired end reads (2 fastq files are generated (R1, R2), is it correct to add...

01 September 2020 7,715 5 View

Does anybody have experience with the detection of a FLAG epitope on a Western blot with the Sigma Anti-FLAG antibody M2 after stripping the blot?

13 August 2020 8,401 3 View

How can I test if my TaqMan Probe is annealing?

Hello! I'm trying to perform a TaqMan qPCR to check for certain fish species in environmental DNA samples. I am using a published primer + probe set. The probe is carrying FAM at it's 5' end and...

14 April 2019 4,907 15 View

Stats for dissertation?

I need some guidance on selecting the appropriate statistical methods for my research. I am investigating lone violent extremists by subtype (far right, far left, Islamic and single-issue) on 4...

11 May 2018 1,809 3 View

If I have cultured keratinocytes expressing only K14 gene, is it possible that under certain conditions, they become activated and express K16?

18 December 2014 1,476 6 View

How I can obtained well formed Embryoid Bodies from Embryonic Stem Cells in Serum free medium?

Hello Everyone I tried to differentiate Embryoid bodies (EBs) from mouse Embryonic Stem cells (E14tg2a) following the protocol I attached. However i failed during the process because despite cells...

01 January 1970 155 6 View

Looking for a psychometric assessment tools to measure maturity in adolescents?

Working on a behavioral therapy protocol and looking for a psychometric assessment tool to measure maturity in adolescents and young adults up to 24 years in age.

01 January 1970 3,684 7 View

How to perform Microsatellite data analysis?

Hello, I have an excel sheet with microsatellite data, I would like to make a NJ tree out of it. I have a basic level in bioinfo. Can someone please guide me through the steps to get the...

01 January 1970 6,828 2 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

What are anisotropic electrical and optical properties of a semiconductor due to?

In some materials, properties such as band gap, thermal conductivity differ along different crystallographic axes. What are the basics?

01 March 2021 1,923 3 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

Why do a weight decrease for thermal degradation in nitrogen gas condition?

Hi all The question is related to TGA(Thermogravimetric analysis). Thermal degradation easily understood but why do samples weight decrease? altough just binding is broked, the weight of...

26 February 2021 1,100 5 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

How to increase solubility of Poly acrylic acid in Dioxane solvent?

I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...

25 February 2021 2,225 5 View

How can I verify my centrifugal fan design for electric motor cooling?

I have radial blade centrifugal fan design which will be mounted on exhaust side of electric traction motor. The fan will be mounted on same motor shaft inside the housing. How can I verify that...

23 February 2021 2,390 3 View

Thermal reduction of graphene oxide.?

I am trying to reduce graphene oxide by thermally but I have no proper information regarding this. Can any one help me regarding the details about temperature and time for the reduction of...

23 February 2021 4,155 4 View

What is the system of units used in the thermal conductivity by Spitzer and Harm?

23 February 2021 9,253 1 View

Which thermal conductivity test recommended for Ag-Cu nanofluid?

I have prepared Ag-Cu alloy with polymer coating upon it.... how can I measure thermal conductivity of it ?

22 February 2021 5,915 3 View