Does anyone have experience on the PhiX concentration required on NovaSeq for ddRAD or RADseq data?

Dear all, we have been running multiple ddRAD libraries prepared by following the protocol of Petersen et al 2014 in our lab on the HiSeq2500 (SR 100) in the past couple of years. We pooled...

28 September 2020 2,297 1 View

Read counts from RNA-Seq paired end reads?

Hello, I have the following question on paired end reads from RNA-seq: To get the total number of obtained reads from paired end reads (2 fastq files are generated (R1, R2), is it correct to add...

01 September 2020 7,715 5 View

Does anybody have experience with the detection of a FLAG epitope on a Western blot with the Sigma Anti-FLAG antibody M2 after stripping the blot?

13 August 2020 8,401 3 View

How can I test if my TaqMan Probe is annealing?

Hello! I'm trying to perform a TaqMan qPCR to check for certain fish species in environmental DNA samples. I am using a published primer + probe set. The probe is carrying FAM at it's 5' end and...

14 April 2019 4,907 15 View

Stats for dissertation?

I need some guidance on selecting the appropriate statistical methods for my research. I am investigating lone violent extremists by subtype (far right, far left, Islamic and single-issue) on 4...

11 May 2018 1,809 3 View

If I have cultured keratinocytes expressing only K14 gene, is it possible that under certain conditions, they become activated and express K16?

18 December 2014 1,476 6 View

How I can obtained well formed Embryoid Bodies from Embryonic Stem Cells in Serum free medium?

Hello Everyone I tried to differentiate Embryoid bodies (EBs) from mouse Embryonic Stem cells (E14tg2a) following the protocol I attached. However i failed during the process because despite cells...

01 January 1970 155 6 View

Looking for a psychometric assessment tools to measure maturity in adolescents?

Working on a behavioral therapy protocol and looking for a psychometric assessment tool to measure maturity in adolescents and young adults up to 24 years in age.

01 January 1970 3,684 7 View

How to perform Microsatellite data analysis?

Hello, I have an excel sheet with microsatellite data, I would like to make a NJ tree out of it. I have a basic level in bioinfo. Can someone please guide me through the steps to get the...

01 January 1970 6,828 2 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View

Can polymer concentrations be calculated based on the number average molecular weight?

Hello, I spin-cast two different polymers onto wafers and measure their height as a function of initial concentration. For polymer A, I have the weight-average molecular weight (Mw) as well as...

28 February 2021 7,882 2 View

How can I publish a manuscript in free or low cost journal with good H-Index?

Dear all I am going to publish my manuscript which is from my thesis in a good H-Index journal but I found them very expensive(I don't have financial support) based on the exchange currency of my...

28 February 2021 7,800 6 View

The Shannon's index is inversely proportional to Simpson's index (1-D)?

I'm having a problem with my bloxplot with Shannon's and Simpson's indexs. The value of Shannon for my Community 1 indicating higher diversity (in relation of the Community 2), but have lower...

26 February 2021 3,033 9 View

Fractional impervious surface (FIS) map ?

Need Help Does anyone Know how to make a Fractional impervious surface (FIS) map from NDVI map, in ArcGIS environment? Thanks in advance

24 February 2021 8,455 2 View

What is the most important parameter of a research work, the impact factor or the h index?

The impact factor is a measure of the importance of a journal based on the number of citations received in a year. ... Articles published in a journal with a high impact factor do not all receive...

24 February 2021 7,481 2 View

How to calculate refractive index (n and kvalue) against energy (in eV) data for alloyed nanomaterials through Independent Phase Approximation method?

Suggest me the method or some other software to calculate the above said data for nanoparticles.

21 February 2021 3,350 7 View

How to get/count refractive index in gasses?

20 February 2021 8,202 6 View

How to realize refractive index values of 1 to 1.1?

Hello researchers! While studying the literature on refractive index sensors, most of the articles simulate their devices with a refractive index ranging from 1 to 1.1, with small steps in...

17 February 2021 1,578 4 View

Any recommendations for methods to analyze elephant space-use (using GPS) and correlating this with changes in NDVI?

For my Masters I am looking into elephant space-use and its effect on vegetation change. I have elephant GPS collar data and was going to use Google Earth Engine to obtain NDVI values. I would...

16 February 2021 7,097 3 View