Hi whats the best way to learn about SDS gel electrophoresis ?

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Mangal Singh

There are many ways to learn about a new technique.

1. You can start with a basic biochemistry book

2. Many videos are available on youtube https://youtu.be/IWZN_G_pC8U

3. To perform a succesful SDS PAGE follow the protocol given in "Molecular Cloning: A Laboratory Manual"

Angel Ashikov

The attached pdf is from Abcam and contains basic knowledge for SDS-PAGE and western blotting. Long time ago I used that one as a guide in my protein research. On their site Abcam provides updated version including video. Similar manuals and guides you can find on the BioRad site any many other SDS-Page suppliers. Usually, technical details of your specific SDS-PAGE system are provided by the manufacturer manual. Good luck.

Rakeshkumar Yadav

please follow the link, it will give you the basic principle.

http://bitesizebio.com/580/how-sds-page-works/

Osama el Ghwail

cheers everybody

still i have problem with how i get start in Lab ?

I am not sure if I got your question right.

Do you have any electrophoretic and WB chambers (for SDS-PAGE and western blot) in your lab? Do you have Acrylamide/Bis-acrylamide solution? Do you have protein standard (protein markers)? You will need reagents to prepare all buffers. If any of these is missing than you should start with buying the right equipment and reagents.