How I become supper Interpretation in science, genetics in cancer research?

I thought which course can make me more understanding and interpret complex in cancer research area before i start any phD program

10 November 2018 2,249 0 View

I am looking for applying PhD position in cancer biology ?

Dear All ... I hope this finds you well, I am from Ireland, I have got MSc in cancer biology and i would like to study PhD somewhere, I would be grateful if I could study under your...

09 October 2018 5,851 0 View

Does anybody known this word meaning Tm ?

I have read a paper about plasmid and PCR then i found this Tm

31 December 2017 5,741 2 View

Any suggestions on how to write good biochemistry literature review ?

Writing a good review article is a real challenge could anyone tell me how to do that

11 December 2017 8,823 2 View

Is there any theoretical handbook about basic Lab tech such as minikit ,transformation and so on ?

i tried to understand some theoretical biochemistry concepts while i am working with the some basic Lab Technics could you please help me out

10 November 2017 6,011 6 View

How do i perform Restriction digestion Pmax cloning vector ?

I am going to do my first restriction digest in the next few days with unequal sites , cut will be in two parts , linear plasmid

10 November 2017 853 0 View

How i do clone with two restriction enzymes BglII and EcoRI into PUc19 plasmid ?

clone gene with BglII and EcoRI flanking into puc19

09 October 2017 2,577 4 View

Have a guidelines and some knowledge but you do not have any exprience in the Lab even your time is not enough to submit your project ?

If you don't have that experience or teacher to help you what should to do

09 October 2017 915 0 View

How I Clone Gene with Hind III and Spe I restriction sides flanking into pUC19 MCS ?

just i asking how i do that theoretically

09 October 2017 7,876 0 View

Hi everyone I looking for short course in related to cancer research coming in one of summer schools around world?

03 April 2017 3,616 0 View

No title

02 March 2021 3,060 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Samples to study circular dichroism (chirality)?

I want to build a low-cost polarimeter to study circular dichroism (chirality). What type of samples can I use? I am looking for samples that can be found outside the laboratory.

01 March 2021 9,101 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View