How I become supper Interpretation in science, genetics in cancer research?

I thought which course can make me more understanding and interpret complex in cancer research area before i start any phD program

10 November 2018 2,249 0 View

I am looking for applying PhD position in cancer biology ?

Dear All ... I hope this finds you well, I am from Ireland, I have got MSc in cancer biology and i would like to study PhD somewhere, I would be grateful if I could study under your...

09 October 2018 5,851 0 View

Any suggestion on which cell culture handbook is the best ?

this is first time i do cell culture in the Lab and i have wondering how i can do that ? where i should start ? which manual could i use ? please help me out

31 December 2017 5,335 1 View

Does anybody known this word meaning Tm ?

I have read a paper about plasmid and PCR then i found this Tm

31 December 2017 5,741 2 View

Any suggestions on how to write good biochemistry literature review ?

Writing a good review article is a real challenge could anyone tell me how to do that

11 December 2017 8,823 2 View

Is there any theoretical handbook about basic Lab tech such as minikit ,transformation and so on ?

i tried to understand some theoretical biochemistry concepts while i am working with the some basic Lab Technics could you please help me out

10 November 2017 6,011 6 View

How i do clone with two restriction enzymes BglII and EcoRI into PUc19 plasmid ?

clone gene with BglII and EcoRI flanking into puc19

09 October 2017 2,577 4 View

Have a guidelines and some knowledge but you do not have any exprience in the Lab even your time is not enough to submit your project ?

If you don't have that experience or teacher to help you what should to do

09 October 2017 915 0 View

How I Clone Gene with Hind III and Spe I restriction sides flanking into pUC19 MCS ?

just i asking how i do that theoretically

09 October 2017 7,876 0 View

Hi everyone I looking for short course in related to cancer research coming in one of summer schools around world?

03 April 2017 3,616 0 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Can anyone provide the Support vector regression code structure of LIBSVM MATLAB or python ?

Please, if Gaussian Parameter optimization is possible in this code structure

28 February 2021 278 3 View

Did anyone ever try using pLVX-TetOne Plasmid with a copGFP as a selection marker instead of Puromycin?

When using a lentiviral vector for inducible expression of genes using the Tet-On system. In the literature, I saw a widely used of pLVX-TetOne-Puro Plasmid, is there a reason why people prefer to...

25 February 2021 1,011 3 View

Why do the PCR bands from the cDNA templates (extraction from the cell after transfection) show different size from the band of the plasmid template?

After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...

25 February 2021 5,712 3 View