I am going to do my first restriction digest in the next few days with unequal sites , cut will be in two parts , linear plasmid
I thought which course can make me more understanding and interpret complex in cancer research area before i start any phD program
10 November 2018 2,382 0 View
Dear All ... I hope this finds you well, I am from Ireland, I have got MSc in cancer biology and i would like to study PhD somewhere, I would be grateful if I could study under your...
09 October 2018 6,052 0 View
this is first time i do cell culture in the Lab and i have wondering how i can do that ? where i should start ? which manual could i use ? please help me out
31 December 2017 5,467 1 View
I have read a paper about plasmid and PCR then i found this Tm
31 December 2017 5,889 2 View
Writing a good review article is a real challenge could anyone tell me how to do that
11 December 2017 8,963 2 View
i tried to understand some theoretical biochemistry concepts while i am working with the some basic Lab Technics could you please help me out
10 November 2017 6,148 6 View
clone gene with BglII and EcoRI flanking into puc19
09 October 2017 2,730 4 View
If you don't have that experience or teacher to help you what should to do
09 October 2017 1,040 0 View
just i asking how i do that theoretically
09 October 2017 8,019 0 View
i might stay alone in next summer i thought that the best way to improve my research skills by attending to short course in next summer any one can advice me please
03 April 2017 3,749 0 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...
05 August 2024 1,573 4 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
Hi, everyone. I want to transfer two genes in to the pseudomonas bacteria that isolated from the soil. The bacteria that I want to use for cloning haven't been identified and not be sequenced,...
02 August 2024 3,987 1 View
Recently I'm trying to use Sep-pack C18 1 cc Vac Cartridge (Waters: SKU: WAT054955) to desalt digested peptides. However, the recovery rate is usually 40-60%. What is the normal recovery rate?
31 July 2024 6,544 11 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello I am trying to create a stable cell line in HEK293 via Lipofectamine 3000 transfection. My plasmid is a CD63-IL10-GFP construct with Puromycin resistance. I am successful with the...
30 July 2024 6,648 1 View
I have been trying to electroporate SKOV3 cells with a large plasmid (11kb) without much success. Any tips?
29 July 2024 3,229 1 View
I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...
28 July 2024 7,559 1 View