just i asking how i do that theoretically
I thought which course can make me more understanding and interpret complex in cancer research area before i start any phD program
10 November 2018 2,382 0 View
Dear All ... I hope this finds you well, I am from Ireland, I have got MSc in cancer biology and i would like to study PhD somewhere, I would be grateful if I could study under your...
09 October 2018 6,052 0 View
this is first time i do cell culture in the Lab and i have wondering how i can do that ? where i should start ? which manual could i use ? please help me out
31 December 2017 5,467 1 View
I have read a paper about plasmid and PCR then i found this Tm
31 December 2017 5,889 2 View
Writing a good review article is a real challenge could anyone tell me how to do that
11 December 2017 8,963 2 View
i tried to understand some theoretical biochemistry concepts while i am working with the some basic Lab Technics could you please help me out
10 November 2017 6,148 6 View
I am going to do my first restriction digest in the next few days with unequal sites , cut will be in two parts , linear plasmid
10 November 2017 994 0 View
clone gene with BglII and EcoRI flanking into puc19
09 October 2017 2,730 4 View
If you don't have that experience or teacher to help you what should to do
09 October 2017 1,040 0 View
i might stay alone in next summer i thought that the best way to improve my research skills by attending to short course in next summer any one can advice me please
03 April 2017 3,749 0 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
Hi, everyone. I want to transfer two genes in to the pseudomonas bacteria that isolated from the soil. The bacteria that I want to use for cloning haven't been identified and not be sequenced,...
02 August 2024 3,987 1 View
Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...
25 July 2024 4,927 3 View
I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...
22 July 2024 5,953 6 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View
Good morning, I am trying to produce a recombinant protein. Unfortunately I one of the two restriction enzymes that I use do not cut at the end of the MCS. After the digestion 15 nucleotides of...
17 July 2024 2,787 4 View
As flaviviruses genome is very frequently prone to mutation hence it is very difficult to clone whole genome inside vector what kind of vector and cells can sustain such amount of cloning...
11 July 2024 4,284 0 View
I am working on cloning of a gene fragment inside my vector everything we tried to do correctly like making insert and vector with utmost care but after ligation the morphology of colonies seems...
11 July 2024 9,010 3 View
This question seeks to address the growing concern of cloned academic journals, which are fraudulent duplicates of legitimate publications. It aims to guide researchers, scholars, and academics on...
10 July 2024 5,966 2 View
Is it appropriate to place the ATG codon in front of the gene of interest, since there is a secretion signal that has its own ATG in front of this gene? I need my protein of interest to be...
10 July 2024 3,050 5 View
