How I become supper Interpretation in science, genetics in cancer research?

I thought which course can make me more understanding and interpret complex in cancer research area before i start any phD program

10 November 2018 2,249 0 View

I am looking for applying PhD position in cancer biology ?

Dear All ... I hope this finds you well, I am from Ireland, I have got MSc in cancer biology and i would like to study PhD somewhere, I would be grateful if I could study under your...

09 October 2018 5,851 0 View

Any suggestion on which cell culture handbook is the best ?

this is first time i do cell culture in the Lab and i have wondering how i can do that ? where i should start ? which manual could i use ? please help me out

31 December 2017 5,335 1 View

Does anybody known this word meaning Tm ?

I have read a paper about plasmid and PCR then i found this Tm

31 December 2017 5,741 2 View

Any suggestions on how to write good biochemistry literature review ?

Writing a good review article is a real challenge could anyone tell me how to do that

11 December 2017 8,823 2 View

Is there any theoretical handbook about basic Lab tech such as minikit ,transformation and so on ?

i tried to understand some theoretical biochemistry concepts while i am working with the some basic Lab Technics could you please help me out

10 November 2017 6,011 6 View

How do i perform Restriction digestion Pmax cloning vector ?

I am going to do my first restriction digest in the next few days with unequal sites , cut will be in two parts , linear plasmid

10 November 2017 853 0 View

How i do clone with two restriction enzymes BglII and EcoRI into PUc19 plasmid ?

clone gene with BglII and EcoRI flanking into puc19

09 October 2017 2,577 4 View

Have a guidelines and some knowledge but you do not have any exprience in the Lab even your time is not enough to submit your project ?

If you don't have that experience or teacher to help you what should to do

09 October 2017 915 0 View

Hi everyone I looking for short course in related to cancer research coming in one of summer schools around world?

03 April 2017 3,616 0 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Sanger Sequencing results not matching experimental results of Restriction Digestion, colony PCR and protein expression?

I have cloned two domains of a gene into two bacterial vectors using cut and paste cloning with different RE sites. I checked the putative clones by colony PCR and Restriction Digestion (fallouts...

18 February 2021 6,736 4 View

Why are Sanger Sequencing reads interrupted in some edited clones?

I have a Crispr/Cas9 edited bulk population and I can briefly check the indel ratios in the bulk population using Sanger sequencing. After cloning single cells, I wanted to check the indel types...

18 February 2021 8,198 6 View

Hi, can anybody help me with the suggestions how can I amplify a stress induced gene of 1107 bp?

I am trying to amplify the TGA1 (Transcription factor 1 ) of Arabidopsis ,which is 1107 bp. I have treated the plants with Salycylic solution over night and extracted the RNA and make cDNA. I...

17 February 2021 7,802 2 View

Alternatives to traditional cloning of insert into backbone (dsDNA)?

Hi, I am stuck with a cloning experiment: The insert & backbone(bb) share one compatible end at 3' end(NotI) and one incompatible end at 5' end (XbaI for insert; HindIII for backbone). So i...

16 February 2021 1,664 3 View

Why do I have trouble with cloning duplicate gene in the same plasmid?

Hello! I am trying to generate a construct in this format: seqA - seqB - linker - seqC - seqB into pSF-CMV-Ub-Puro-Ascl plasmid 0.5kb 2kb 66 0.25kb 2kb I have tried many...

15 February 2021 7,118 3 View

Would using an asRNA for repressing transcription of a high expression Listeria monocytogenes non-coding RNA harbored in a plasmid be a viable option?

Hello all, I am interested in deleting a highly expressed non-coding RNA located on a Listeria monocytogenes plasmid. Currently, there are not any optimized gene deletion methods for Listeria...

09 February 2021 4,994 1 View