Does anybody known this word meaning Tm ?

More Osama el Ghwail's questions See All

How I become supper Interpretation in science, genetics in cancer research?

I thought which course can make me more understanding and interpret complex in cancer research area before i start any phD program

10 November 2018 2,382 0 View

I am looking for applying PhD position in cancer biology ?

Dear All ... I hope this finds you well, I am from Ireland, I have got MSc in cancer biology and i would like to study PhD somewhere, I would be grateful if I could study under your...

09 October 2018 6,052 0 View

Any suggestion on which cell culture handbook is the best ?

this is first time i do cell culture in the Lab and i have wondering how i can do that ? where i should start ? which manual could i use ? please help me out

31 December 2017 5,467 1 View

Any suggestions on how to write good biochemistry literature review ?

Writing a good review article is a real challenge could anyone tell me how to do that

11 December 2017 8,963 2 View

Is there any theoretical handbook about basic Lab tech such as minikit ,transformation and so on ?

i tried to understand some theoretical biochemistry concepts while i am working with the some basic Lab Technics could you please help me out

10 November 2017 6,148 6 View

How do i perform Restriction digestion Pmax cloning vector ?

I am going to do my first restriction digest in the next few days with unequal sites , cut will be in two parts , linear plasmid

10 November 2017 994 0 View

How i do clone with two restriction enzymes BglII and EcoRI into PUc19 plasmid ?

clone gene with BglII and EcoRI flanking into puc19

09 October 2017 2,730 4 View

Have a guidelines and some knowledge but you do not have any exprience in the Lab even your time is not enough to submit your project ?

If you don't have that experience or teacher to help you what should to do

09 October 2017 1,040 0 View

How I Clone Gene with Hind III and Spe I restriction sides flanking into pUC19 MCS ?

just i asking how i do that theoretically

09 October 2017 8,019 0 View

Hi everyone I looking for short course in related to cancer research coming in one of summer schools around world?

i might stay alone in next summer i thought that the best way to improve my research skills by attending to short course in next summer any one can advice me please

03 April 2017 3,749 0 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Where should the ARS sequence be introduced on a plasmid?

Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...

05 August 2024 1,573 4 View

"A Markov-like Model for Patient Progression"?

A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...

05 August 2024 10,079 0 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Identify the eight key processes of excellent supply chain management and discuss how each of these processes impacts the end customer?

Read the journal article by Douglas M. Lambert, “The Eight Essential Supply Chain Management Processes,” Supply Chain Management Review, Vol. 8, No. 6 (2004), pp. 18-26

04 August 2024 9,919 4 View

Does atmosphere affect flow of matter & energy on Earth & flow of energy in biosphere related to the flow of food through a food chain?

How does the atmosphere affect the flow of matter and energy on Earth and flow of energy in the biosphere related to the flow of food through a food chain?

02 August 2024 9,644 0 View