I have read a paper about plasmid and PCR then i found this Tm
It stands for melting temperature of a primer or pcr product when half of the DNA duplex will become single stranded.
thank you Michael i have seen like Tm or T with m but over down the line and then got confused ~! thank you anyway ...
I thought which course can make me more understanding and interpret complex in cancer research area before i start any phD program
10 November 2018 2,249 0 View
Dear All ... I hope this finds you well, I am from Ireland, I have got MSc in cancer biology and i would like to study PhD somewhere, I would be grateful if I could study under your...
09 October 2018 5,851 0 View
this is first time i do cell culture in the Lab and i have wondering how i can do that ? where i should start ? which manual could i use ? please help me out
31 December 2017 5,335 1 View
Writing a good review article is a real challenge could anyone tell me how to do that
11 December 2017 8,823 2 View
i tried to understand some theoretical biochemistry concepts while i am working with the some basic Lab Technics could you please help me out
10 November 2017 6,011 6 View
I am going to do my first restriction digest in the next few days with unequal sites , cut will be in two parts , linear plasmid
10 November 2017 853 0 View
clone gene with BglII and EcoRI flanking into puc19
09 October 2017 2,577 4 View
If you don't have that experience or teacher to help you what should to do
09 October 2017 915 0 View
just i asking how i do that theoretically
09 October 2017 7,876 0 View
03 April 2017 3,616 0 View
Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...
02 March 2021 4,029 3 View
Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....
02 March 2021 285 2 View
So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...
02 March 2021 1,146 2 View
Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...
01 March 2021 210 1 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...
01 March 2021 9,310 4 View
To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...
01 March 2021 8,683 4 View
Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?
01 March 2021 5,311 1 View
I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...
28 February 2021 5,440 3 View
I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...
28 February 2021 4,949 3 View