Hi, is there is a mouse immortal NK cell line that you would recommend?

I need to use mouse NK cells for my research...usually I use primary NK cells from the mice's spleen but I have to kill large number of mice (SJL) to get good number of NKs... thus I want to use...

11 December 2019 3,637 3 View

Why the scramble siRNA (that is used in negative control) gives result different from the result of untransfected cells?

04 May 2017 7,383 2 View

Hi, why in non-dividing cells the silencing effect of a gene by siRNA is lost after few weeks?

I know in rapidly dividing cells, the silencing of a gene by siRNA is lost because it gets diluted each time the cells divide...but in case of nondividing cells why it doesn't last till the cell...

01 February 2017 7,747 3 View

Hi, why scrambled siRNA is used in negative control cells when doing siRNA transfection?

31 December 2016 5,428 5 View

Hi, why the sequencing peaks for the same nucleotide base (ex: for Adenine (green) in the attached picture) differ in their heights ?

Aren't they supposed to be the same height for the same nucleotide base?

09 October 2016 8,700 5 View

Hi, how can the monoclonal antibody that binds to antigen/receptor present in both normal and malignant cells, targets the malignant only?

04 May 2016 9,604 8 View

Why RNA and DNA have the same nucleotide bases except for Uracil?

02 March 2016 2,593 3 View

How the mutation, or the polymorphism, that is located in the non coding region (intron) can cause diseases ?

11 December 2015 7,108 3 View

Hi..Why we need high pH for the resolving gel in SDS-PAGE?

How does the pH exactly affect the movement of the protein samples?

10 November 2015 6,693 3 View

Hi..I want to ask why only dendritic cells are modified in order to make T cells act against the tumor cells' markers and antigens?

why they don't genetically modify the T cells directly to make them produce receptors and antibodies specific for these antigens?

08 September 2015 950 2 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

No title

02 March 2021 3,060 3 View

Is there a powerful system for the security of the systems distributed on IoT systems?

Is there a powerful system for the security of the systems distributed on IoT systems?

02 March 2021 3,858 10 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

How Relativistic effects is one reason for lanthanide contraction?

The Lanthanide contraction which is the decrease in ionic radii of the elements in the lanthanide series from atomic number 57, lanthanum, to 71, lutetium, is due to poor shielding of the 4f...

01 March 2021 2,272 4 View