What about XMRV retrovirus immortalization kit and it is efficiany rate? Do you recommed another kit...
Thanks in advance
https://www.creative-bioarray.com/products/cell-immortalization-kit-list-202.htm?page=1
I need to use mouse NK cells for my research...usually I use primary NK cells from the mice's spleen but I have to kill large number of mice (SJL) to get good number of NKs... thus I want to use...
11 December 2019 3,804 3 View
why the production of a protein by cells transfected with siRNA (in-vitro) is less than the production of that protein by untransfected cells? Thanks in advance
04 May 2017 7,581 2 View
I know in rapidly dividing cells, the silencing of a gene by siRNA is lost because it gets diluted each time the cells divide...but in case of nondividing cells why it doesn't last till the cell...
01 February 2017 7,899 3 View
What is the use of doing siRNA transfection with NC scrambled siRNA for negative control cells? Why we just don't transfect them since they are just a negative control? Thanks
31 December 2016 5,688 5 View
Aren't they supposed to be the same height for the same nucleotide base?
09 October 2016 8,820 5 View
As I was preparing the attached paper, it's mentioned that CD20 receptor is expressed in both normal and lymphoid cancer cells (page # 3, highlighted), yet, they designed monoclonal antibodies...
04 May 2016 9,727 8 View
Why they differ in Uracil specifically and not other base?
02 March 2016 2,725 3 View
how they can affect the expression of the encoded proteins?
11 December 2015 7,246 3 View
How does the pH exactly affect the movement of the protein samples?
10 November 2015 6,815 3 View
why they don't genetically modify the T cells directly to make them produce receptors and antibodies specific for these antigens?
08 September 2015 1,047 2 View
I want to explore the long-term effects of incarceration on a youth's developing brain. I also want to explore research that looks critically at incarceration and punitive measures as the primary...
12 August 2024 862 0 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
What are the emerging trends and opportunities for STEM education and careers in the online space?
10 August 2024 507 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.
08 August 2024 3,746 4 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View