Hi, is there is a mouse immortal NK cell line that you would recommend?

I need to use mouse NK cells for my research...usually I use primary NK cells from the mice's spleen but I have to kill large number of mice (SJL) to get good number of NKs... thus I want to use...

11 December 2019 3,637 3 View

Hi, what is the best immortalization kit for mouse primary cells?

What about XMRV retrovirus immortalization kit and it is efficiany rate? Do you recommed another kit... Thanks in advance

11 December 2019 2,704 1 View

Why the scramble siRNA (that is used in negative control) gives result different from the result of untransfected cells?

04 May 2017 7,383 2 View

Hi, why in non-dividing cells the silencing effect of a gene by siRNA is lost after few weeks?

I know in rapidly dividing cells, the silencing of a gene by siRNA is lost because it gets diluted each time the cells divide...but in case of nondividing cells why it doesn't last till the cell...

01 February 2017 7,747 3 View

Hi, why scrambled siRNA is used in negative control cells when doing siRNA transfection?

31 December 2016 5,428 5 View

Hi, why the sequencing peaks for the same nucleotide base (ex: for Adenine (green) in the attached picture) differ in their heights ?

Aren't they supposed to be the same height for the same nucleotide base?

09 October 2016 8,700 5 View

Hi, how can the monoclonal antibody that binds to antigen/receptor present in both normal and malignant cells, targets the malignant only?

04 May 2016 9,604 8 View

Why RNA and DNA have the same nucleotide bases except for Uracil?

02 March 2016 2,593 3 View

How the mutation, or the polymorphism, that is located in the non coding region (intron) can cause diseases ?

11 December 2015 7,108 3 View

Hi..Why we need high pH for the resolving gel in SDS-PAGE?

How does the pH exactly affect the movement of the protein samples?

10 November 2015 6,693 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

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03 March 2021 5,360 2 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View