Hi, is there is a mouse immortal NK cell line that you would recommend?

I need to use mouse NK cells for my research...usually I use primary NK cells from the mice's spleen but I have to kill large number of mice (SJL) to get good number of NKs... thus I want to use...

11 December 2019 3,637 3 View

Hi, what is the best immortalization kit for mouse primary cells?

What about XMRV retrovirus immortalization kit and it is efficiany rate? Do you recommed another kit... Thanks in advance

11 December 2019 2,704 1 View

Why the scramble siRNA (that is used in negative control) gives result different from the result of untransfected cells?

04 May 2017 7,383 2 View

Hi, why in non-dividing cells the silencing effect of a gene by siRNA is lost after few weeks?

I know in rapidly dividing cells, the silencing of a gene by siRNA is lost because it gets diluted each time the cells divide...but in case of nondividing cells why it doesn't last till the cell...

01 February 2017 7,747 3 View

Hi, why scrambled siRNA is used in negative control cells when doing siRNA transfection?

31 December 2016 5,428 5 View

Hi, why the sequencing peaks for the same nucleotide base (ex: for Adenine (green) in the attached picture) differ in their heights ?

Aren't they supposed to be the same height for the same nucleotide base?

09 October 2016 8,700 5 View

Hi, how can the monoclonal antibody that binds to antigen/receptor present in both normal and malignant cells, targets the malignant only?

04 May 2016 9,604 8 View

How the mutation, or the polymorphism, that is located in the non coding region (intron) can cause diseases ?

11 December 2015 7,108 3 View

Hi..Why we need high pH for the resolving gel in SDS-PAGE?

How does the pH exactly affect the movement of the protein samples?

10 November 2015 6,693 3 View

Hi..I want to ask why only dendritic cells are modified in order to make T cells act against the tumor cells' markers and antigens?

why they don't genetically modify the T cells directly to make them produce receptors and antibodies specific for these antigens?

08 September 2015 950 2 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View