Hi there, I am having difficulty in doing western blotting of MBL serum protein. I would appreciate if someone could share detail protocol for WB ?

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Arseniy Yuzhalin

try this protocol:

http://www.abcam.com/index.html?pageconfig=resource&rid=11375&source=pagetrap&viapagetrap=westernblotguide

Bizaya Malla

Hi Arseniy,

Thanks for sharing the link. I know WB well but for some unknown reasons, my WB for MBL2 is not working. So, I appreciate a specific protocol for it, if anyone is also working on it.

I`m not sure there`s specific protocol for just one protein. Why doesn`t your WB work?