Western blot
How do I measure VDR expression in HepG2 cell lines? Is immunohistochemistry the most appropriate way?
31 December 2014 7,804 1 View
Can anyone share protocols on western blot of MBL2 expression from HepG2 cell lines ?
11 December 2014 390 0 View
I am using 1:10 dilution of serum sample after heating for 5 minutes at 99C.
09 October 2014 8,156 3 View
I am running WB for my protein of interest. should I use loading control together with sample during WB step or I have to do it after stripping ?
08 September 2014 4,473 5 View
I added loading buffer of 2ul with DTT, and Bromophenol blue with 10ul of serum, but serum solidified after 5 minutes at 95C heating.
08 September 2014 8,127 2 View
I have SNPs information for 10 loci of a gene and not the sequence in FASTA. Which software is better in such situation when I have only SNPs call in the excel sheet?
08 September 2014 487 1 View
Western blot, serum sample
08 September 2014 2,114 2 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Hi, I have lysate from human macrophages, and I wonder what will be the best way to check the NFKB activation in those cells. I was thinking p-p65 or p-ikkb./ Is that make sense?
18 July 2024 5,214 4 View
Having trouble detecting phosphorylated HSL. Any tips for how to prevent de-phosphorylation events or detect phosphorylation?
15 July 2024 2,055 3 View
I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...
15 July 2024 6,668 0 View
I know mass spectrometry will work. But, is there any simple and straightforward method to distinguish between the two proteins of the same molecular weight? They are cryptochromes and...
14 July 2024 9,324 2 View
Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...
12 July 2024 6,313 0 View
Hello, I isolated total histones successfully from the skin primary cells by acid extraction. I wanted to detect histone H1.2 expression in different conditions by western blot. Can some one...
10 July 2024 9,165 1 View
Hello! I am planning a western blot experiment, and I am looking for GAPDH antibodies to order. In the past, I have experienced a lot of hit-or-miss when ordering antibodies based on just the...
07 July 2024 1,685 0 View
I have this problem with my western blot for the ABCG2 antibody on nitrocellulose membrane. Help please
02 July 2024 8,715 2 View
