How do I measure VDR expression in HepG2 cell lines? Is immunohistochemistry the most appropriate way?
I totally agree with Theocharis as these are also my suggestions. Best methods are immunohistochemitry and QPCR for protein and gene expression, respectively.
Wishes,
Bassem
Can anyone share protocols on western blot of MBL2 expression from HepG2 cell lines ?
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I am using 1:10 dilution of serum sample after heating for 5 minutes at 99C.
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Western blot, serum sample
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Western blot
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