Bizaya Malla

8 Questions 3 Answers 0 Followers

Questions related from Bizaya Malla

How do I measure VDR expression in HepG2 cell lines?

How do I measure VDR expression in HepG2 cell lines? Is immunohistochemistry the most appropriate way?

01 January 2015 7,798 1 View

Can anyone share protocols on western blot of MBL2 expression from HepG2 cell lines ?

12 December 2014 385 0 View

Hi there, I am having difficulty in doing western blotting of MBL serum protein. I would appreciate if someone could share detail protocol for WB ?

I am using 1:10 dilution of serum sample after heating for 5 minutes at 99C.

10 October 2014 8,151 3 View

How the software is better for linkage disequilibrium with only SNPs data in the excel sheet?

I have SNPs information for 10 loci of a gene and not the sequence in FASTA. Which software is better in such situation when I have only SNPs call in the excel sheet?

09 September 2014 482 1 View

What can be used as loading control or positive control for MBL western blot ?

Western blot, serum sample

09 September 2014 2,109 2 View

When to use loading control during Western blot ?

I am running WB for my protein of interest. should I use loading control together with sample during WB step or I have to do it after stripping ?

09 September 2014 4,466 5 View

How do I do a serum dilution so that I get 20ug of protein for western blot ?

I added loading buffer of 2ul with DTT, and Bromophenol blue with 10ul of serum, but serum solidified after 5 minutes at 95C heating.

09 September 2014 8,122 2 View

What is the use of Loading control in western blot ? Can anyone share protocol for the use of GAPDH in western blot as loading control ?

Western blot

09 September 2014 1,573 0 View