How do I measure VDR expression in HepG2 cell lines?

31 December 2014 7,775 1 View

Can anyone share protocols on western blot of MBL2 expression from HepG2 cell lines ?

Can anyone share protocols on western blot of MBL2 expression from HepG2 cell lines ?

11 December 2014 363 0 View

Hi there, I am having difficulty in doing western blotting of MBL serum protein. I would appreciate if someone could share detail protocol for WB ?

I am using 1:10 dilution of serum sample after heating for 5 minutes at 99C.

09 October 2014 8,132 3 View

When to use loading control during Western blot ?

I am running WB for my protein of interest. should I use loading control together with sample during WB step or I have to do it after stripping ?

08 September 2014 4,442 5 View

How the software is better for linkage disequilibrium with only SNPs data in the excel sheet?

08 September 2014 460 1 View

How do I do a serum dilution so that I get 20ug of protein for western blot ?

I added loading buffer of 2ul with DTT, and Bromophenol blue with 10ul of serum, but serum solidified after 5 minutes at 95C heating.

08 September 2014 8,099 2 View

What is the use of Loading control in western blot ? Can anyone share protocol for the use of GAPDH in western blot as loading control ?

Western blot

08 September 2014 1,546 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What are some important techniques for protein induction and SDS-PAGE analysis?

Hello everyone. I am fairly new in protein chemistry field. I am trying to perform a protein induction hoping to purify my protein of interest using FPLC method. However, I am having trouble on...

03 March 2021 8,237 3 View

SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View