Is this method mentioned for sporulation assessment reliable ?

I had come across a protocol to assess and score the sporulation of fungi, where they has taken out a 5 mm fungal disc and transferred to 5ml sterile dist.water and spore count was recorded under...

05 January 2021 9,468 1 View

Easy and effective method to visualize fungal mycelium inside leaf tissue?

Is there any easy and efficient method to visualize the fungal mycelium inside infected leaf tissue using light microscoopy? Please share protocols which can be performed using limited resources...

03 January 2021 4,217 2 View

What is the best way to maintain constant concentration of a volatile organic liquid in an open experimental set-up?

The purpose is to keep a NAPL (eg Toluene) concentration constant in an open tank set-up.

27 December 2020 7,325 2 View

How to calculate width and length of the tapered microstrip line feed of a SIW leaky wave antenna?

With reference to substrate integrated waveguide -leaky wave antenna

26 December 2020 3,982 2 View

How can I extract radial stress from cartesian coordinate system in COMSOL?

Hello, I have a simulation in COMSOL which is a beam that is under bending and I don't know how could I extract radial stress of it from cartesian components. Is there anyone who may help me?

23 December 2020 8,408 2 View

Don't you have medical quacks claiming that they can cure COVID in your countries?

There's a quack who claims that he can cure patients with COVID using his 'magic portion'. Some media blindly support him, though there's no scientific evidence for his treatment. There's a...

17 December 2020 8,022 5 View

Review the debate; brain and mind. Can the mind exist without the brain?

Brain vs mind is a never solved debate. A school of thought is that the mind can function without the brain. Is it possible?

10 December 2020 2,618 4 View

Is mind travel possible?

If you read buddhist scriptures, you can find a number of stories about mind travel. It indicates that lord buddha used to mind travel to far away places and interacted with people. What do you...

30 November 2020 3,314 19 View

What will be a futuristic city look like?

The extent of usable land will decline due to rise in sea levels. Many islands will be disappeared. People might have to carry oxygen tanks to breathe. Technological development won't go any...

29 November 2020 6,276 4 View

How to determine presence of Cross Sectional Dependence in Panel data?

Hello everyone. I have a panel data of N=30 and T=20. It's not exactly a case of small T and large N. Can I use the command xtcsd, pesaran in stata to check for cross sectional dependence? I am a...

07 October 2020 7,676 13 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

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22 February 2021 9,932 11 View

Is there a method for evaluating nanoparticle adhesion to epithelial tissue in vitro?

I want to measure how well nanoparticles can transfect cells based on NP adhesion properties. In the literature I find people do it in animal studies, and epithelial adhesion helps in transfection...

21 February 2021 7,791 3 View

Are exogenous molecules transferred between human cells during doubling ?

Hello RG community, I wonderer whether exogenous molecules (e.g. reporter plasmids, miRNAs or other artificial molecules) are transferred between cultured human cells during their doubling ? So,...

21 February 2021 6,633 3 View

How to select therorical or conceptual framework for any proposed qualitative research work?

Hi, I am conducting an exploratory research with qualitative in-depth interview approach to understand the impact of COVID_19 in health systems response to burn care in India. Since, this is a...

19 February 2021 4,562 4 View

How to model rupture in geogrid in abaqus?

Hi researchers I wanna model tensile test on geogrid in abaqus, so I added displacement to one side of geogrid and the other side of geogrid is constrained completely, everything seems normal but...

19 February 2021 2,195 2 View

Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View