Please share the GC-MS sample preparation of algal biomass. My research is focused and interested in lipid content should I go for whole biomass or extract lipid and then perform the FAME analysis what would be more efficient and better?
1. Get the known volume of biomass. ( if you want to take lipid from wet biomass)
2. dry it (if you want to take lipid from dry biomass)
3. Mix solvents for polar and non-polar lipids (as you wish)
4. Extract lipid and convert into FAME by transesterification
best wishes
you can follow my papers already there in RG
you have to first extract lipid and then need to prepare fatty acid methyl ester (FAME)
You can follow the paper mentioned below:
1. Article Microalgae harvest influences the energy recovery: A case st...
2. Article Lipid extraction and analysis of microalgae strain pectinode...
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