Hy,
I want to express my gene in in-vitro cell culture. So which expression vector is best.
Thanks,
Regard,
Muhammad Rashid
Hi Muhammad,
Actually there are a lot of things to consider before chosing a suitable vector:
For example:
a) in which host do you want to express your gene?
b) do you need any Tags for detection or purification?
Also most of the time such a thing as the perfect vector does not exist, moreover you should find the vector that suits your needs in the best possible way
Best,
Pascal
There is not a perfect vector for any experiment/application. To choose a vector it is important to consider the type of cells to transfect, the requirements for expression (strong expression in a short time for instance, constitutive or inducible expression, etc.) , the transfection method, etc.
I think you should post again the question giving more details on your experimental system and your requirements in order to have some advices.
Hello,
First of all you will have to be very specific about the type of cell culture you are performing and what gene do you want to encode and express in the particular cells. When it comes to expression vector there is a long list of vectors available that would suit your purpose. But more importantly do consider the following:
1. The type of ells being cultured (mammalian, or insect cells, or plant cells)
2. The size and function of the gene of interest to be transfected.
3. The ability of the cell culture to express the protein of interest in the correct conformational structure as per your requirement to study its exact function.
All the Best!
Akshay
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