Amphyphilic compound Supplier and product ID?

Dear Expert, I want to know the Amphyphilic compound that can expand the phospholipid bilayer of animal cell membrane. Below attachment is the reference paper where they use this product but no...

08 September 2018 6,486 2 View

Suitable vector for transfection of Theileria annulata cell line?

Dear Expert, I want to express my gene of interest in Theileria annulata cell line. I have pcDNA3.1/myc-His(-) A vector. Is this possible to express that gene with this vector or I may choose any...

07 August 2018 7,540 1 View

Hi Experts, Which vector is best for transfection?

Hy, I want to express my gene in in-vitro cell culture. So which expression vector is best. Thanks, Regard, Muhammad Rashid

04 May 2018 8,069 4 View

Hi Experts, Which vector is best for transfection?

Basically we want to express a gene in theileria transformed cell line for its interaction on the function of transformed cells. For transfection, we will use electroporation. So that vector will...

04 May 2018 2,134 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

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28 February 2021 9,936 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View