LPS work better in normal conc. of FBS or reduced?

Dear experts, During in-vitro culture of cells, LPS works better in normal conc. of FBS or reduce in culture medium? usually I use 10% FBS. Either I use this conc. of FBS or reduce it for...

31 December 2018 8,958 2 View

Suitable vector for transfection of Theileria annulata cell line?

Dear Expert, I want to express my gene of interest in Theileria annulata cell line. I have pcDNA3.1/myc-His(-) A vector. Is this possible to express that gene with this vector or I may choose any...

07 August 2018 7,540 1 View

Hi Experts, Which vector is best for transfection?

Hy, I want to express my gene in in-vitro cell culture. So which expression vector is best. Thanks, Regard, Muhammad Rashid

04 May 2018 8,069 4 View

Hi Experts, Which vector is best for transfection?

Basically we want to express a gene in theileria transformed cell line for its interaction on the function of transformed cells. For transfection, we will use electroporation. So that vector will...

04 May 2018 2,134 2 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

Mediation path analysis - Can I run two seperate models?

I am wanting to run a serial mediation model with 2 x IVs, 2 x MVs, and 3 DPs (all continuous). Is it problematic to run 2 different models for each IV, keeping the MVs and DPs the same in each...

27 February 2021 5,681 3 View