I had run the sample in SDS-PAGE and obtained gel bands, additionally i am interested to know that how to calculate the protein purification level of protein run in SDS-PAGE.
If you are using an instrument to take a picture of the gel, it likely has an option to measure band intensity/volume. Measure the intensity of each band, you can relate these values to purity.
Having more than one band per sample indicates that a protein was not pure. I agree with what Emil suggested and it is better to run a known pureed protein with your sample to be used later as a purity reference. Good luck
Run the Known pure protein with your sample(s) then you can measure the purity of your samples with your reference sample/ control
1)In PAGE gel picture using image-J or other software, quantify the purified protein's band intensity which is divided by Total lane intensity multiplied by 100. (Test protein intensity/ total lane intensity)*100
2) Compare with commercial BSA or available pure protein in your lab, for workable purity or for gel based absolute quantification.
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