CHANDRA SHEKHAR DASARI

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Questions related from CHANDRA SHEKHAR DASARI

Amplification of constitutive gene and target in same pcr tube can be performed or not ? why not we do RT-PCR like western(same lane with two bands)?

Can we do amplification of our target along with constitutive gene in the same tube(both sets of primers in same tube) when their lengths are resolvable ? If yes why it is not practicable(/this...

05 May 2015 9,969 1 View

Has anyone used restriction enzymes for cloning which produce one sticky end and one blunt end?

I only have two possible restriction sites for cloning an insert into my vector. One end is a sticky end (bglII) and the other end is a blunt end (HPA1or KSPA1). I have never done a blunt end...

01 January 2015 4,062 10 View

MMP-2 bands are giving streak lines across the gel.

I attached the zymogram image. The top line is MMP-9 giving distinct band. But the MMP2 band is like a straight line across the gel both latent and active forms even in the marker lane. Could any...

04 April 2014 4,370 6 View

What are the dot particles in my LNCaP cell culture?

I found some dot particles after reviving. They have not washed away after 2 to 3 passages. The media colour is not changing even though the particles are huge. The particles are not moving. I...

03 March 2014 5,665 10 View

What is the amount of protein we can expect from T25 flask?

I need at least 100ug of total protein of LNCaP cells. Can I use T25 flask with 60-70% confluency stage or do I need to wait until it becomes more confluent? How much RIPA is preferable for lysis?

10 October 2013 2,142 1 View