Hi, does dissolving polar compounds in water change its dielectric constants/polarity?

More Lora Altahrawi's questions See All

Why is my protein assay standard curve bended?

Hello everyone, I wish to measure the total protein concentration of some samples but my standards are always bended rather than giving a nice straight line. I first tried to make the standards...

09 August 2023 3,305 7 View

Oxytocin plasmids for cell transfection?

Dear All, I am currently looking for a plasmid (or paper using a plasmid) of oxytocin to transfect it to cell lines. On Addgene, I only found viral plasmids and if I can avoid that, that would...

05 June 2023 8,658 0 View

Does multiple stimulation with DHPG are effective ?

Hi everyone ! I am performing acute slice Ca-imaging and I would like to register the fluo rate increase on neurons in response to DHPG( group I mGlu receptor agonist). Does somenone know if I...

25 February 2019 7,932 1 View

Identify oxytocin neuron in living hypothlamaic slices ?

Hello, does anyone know a way to identify oxytocin neuron over the vasopressin neuron in acute slices ? I am performing calcium imaging in the PVN of mice with Calbryte dye and recording calcium...

19 November 2018 9,653 3 View

Cuáles autoras son imprescindibles para el estudio de las mujeres en los medios de comunicación?

En nuestra última sesión de seguimiento, se me señaló que era importante incluir a las autoras más recientes sobre la participación de la mujer en los medios de comunicación.

14 September 2018 8,364 0 View

How to measure the solubility of one compound in a mixture of organic compounds?

(ethyl acetate)

18 August 2017 2,022 3 View

Markers to detect human or mouse myeloid suppressor cells (MSDC) by Immunohistology and flow cytometry

I need antibodies to detect MSDC by immunohistology and flow cytometry in cancer infiltrate.

23 January 2013 9,087 3 View

Markers to detect human or mouse myeloid suppressor cells (MSDC) by Immunohistology and flow cytometry

I need antibodies to detect MSDC by immunohistology and flow cytometry in cancer infiltrate.

31 December 2012 9,365 3 View

Vasopressin half-life

Hello everyone, By searching the half-life of vasopressin, it appears that there are huge fluctuations between studies... Some demonstrate that, in blood, the half-life is about 2-3 min whereas...

01 January 1970 4,443 3 View

Which software to analyse fluorescent imaging?

Dear All, Apart from ImageJ, which software do you use to analyse light microscopy images? To do basic things like colocalization analysis, measure of fluorescence increase/decrease against...

01 January 1970 9,580 3 View

How does grain and grain boundary affect the ceramic when studying its dielectric properties?

I am not able to get good literature and the physics behind how first these grains and grain boundaries arises out of no where when we make a pellet to study its dielectric properties and then how...

07 August 2024 5,177 3 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

Determining the worth of a point improvement in Hamilton Depression Scale?

Dear readers, Thanks for your attention. I am wondering about the health economic problem of quantifying the value of interventions which a) prevent, b) improve symptom profile and c) ultimately...

05 August 2024 3,246 1 View

Why do paired electrons atoms produce no magnetic field lines?

If you think of electrons with spin as bar magnets, you know bar magnets of opposite polarity as long as they're not occupying the same spatial location don't cancel out each other's magnetic...

03 August 2024 3,927 2 View

Conjugation of PEG-Amine to an Amino Acid Using EDC?

I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...

31 July 2024 2,033 1 View

How to calculate conductivity using Electrochemical Impedance Spectroscopy's Nyquist plot?

Hello everyone, I am new to this characterization but watching videos on youtube I have already plotted a Nyquist plot (Z' and -Z'') and already fitted models on my material using Zsim. But now I...

29 July 2024 1,376 0 View

Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?

Hi, researchers, Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?

29 July 2024 4,570 0 View

My cfDNA extraction protocol using silica beads works with plasma collected in COD SAGM bags but not with plasma collected in K2 EDTA tubes. Why so?

I am currently performing cell-free DNA (cfDNA) extraction from plasma using an in-house developed kit that employs silica-coated magnetic beads. My protocol works effectively with plasma obtained...

28 July 2024 3,559 0 View

What is the Principle of Cysteine-carbazole-sulfuric acid reaction with glucose?

I have been looking for the principle of Cysteine-carbazole-sulfuric acid reaction in research papers and books, but couldn't find it. This reaction is mainly used for the quantification of...

23 July 2024 3,891 0 View

Why methanol and sulphuric acid used in the analysis of polyhydroxyalkanoates (PHA) by GC-MS?

Why methanol and sulphuric acid, used in the analysis of polyhydroxyalkanoates (PHA) methyl esters by GC-MS? Additionally, why do we typically use non-polar solvents in GC-MS?

22 July 2024 1,210 2 View

Saeid Maghsoudi

Hope you are doing fine.

When a polar compound dissolve in water, it maybe depends on derivatives which cuases hydrogen bonding and this is one of the reason that can impact on dielectric polarity.

As you have amino acids in your project, you will face with this issue.

Best regards

Lora Altahrawi

appreciate it thanks

Bojidarka B. Ivanova

Mrs/miss Altahrawi,

Yes it does.

appreciate it thanks :)