Hello, does anyone know a way to identify oxytocin neuron over the vasopressin neuron in acute slices ? I am performing calcium imaging in the PVN of mice with Calbryte dye and recording calcium spikes but I don't know how to diffrentiate the responses from the OT neurons over the VP neurons.
As I'm working on specific KO mice, I can't use GFP-OT mice...
Thank you for your help !
Dear Lora,
have a look on the paper from Charlet's team
A New Population of Parvocellular Oxytocin Neurons Controlling Magnocellular Neuron Activity and Inflammatory Pain Processing.
Eliava M, Melchior M, Knobloch-Bollmann HS, Wahis J, da Silva Gouveia M, Tang Y, Ciobanu AC, Triana Del Rio R, Roth LC, Althammer F, Chavant V, Goumon Y, Gruber T, Petit-Demoulière N, Busnelli M, Chini B, Tan LL, Mitre M, Froemke RC, Chao MV, Giese G, Sprengel R, Kuner R, Poisbeau P, Seeburg PH, Stoop R, Charlet A, Grinevich V.
Neuron. 2016 Mar 16;89(6):1291-1304.
Article A New Population of Parvocellular Oxytocin Neurons Controlli...
more specifically fig 2 for electrophysiological identification.
Dear Pascal,
Thank you for your answer and for the paper.
The trouble is that I am not patching the cells. I can't perform electrophysiological recording in the same time of imaging and I didn't find a way (for now) to identify the OT neurons without patching them... Maybe a kind of marker I can use simultaneously with the calcium dye exist but I didn't find it yet.
In Charlet team they are also using viral tracer to identify OT cells. Check the last paper published.
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