Hi everyone !
I am performing acute slice Ca-imaging and I would like to register the fluo rate increase on neurons in response to DHPG(
group I mGlu receptor agonist). Does somenone know if I can stimulate the same slice multiple times with DHPG ? Or does only the first stimulation is reliable ?
I obtain very variable responses but I don't know if it's correlated with the number of stimulations or if the responses are classically very variable in this experiment...
Thank you for your help
Good point Lora.
Sorry I do not have nore comment that this. My research follow the way of a Plan Electrophysiologist. I am not good enough in Organic Chemistry. However, just I am studying Synapse on Neural Network System. I am very passionate into understand, in detail, who electricity (electrones) change into chemicals from the neuron transporter to the neuron receptor.
Let us continue our conversations. We PhD students have to support each other in the usually funny way. That is life, isn't it?
Cheer,
Victor P ROJAS Yupanqui
ISA STAR-C UNI PERU
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